Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Slovakia.
Department of Health Psychology and Research Methodology, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Slovakia.
Physiol Rep. 2021 May;9(10):e14879. doi: 10.14814/phy2.14879.
Cardiovascular comorbidities are independent risk factors for mortality in dialysis patients. MicroRNA signaling has an important role in vascular aging and cardiac health, while physical activity is a primary nonpharmacologic treatment for cardiovascular comorbidities in dialysis patients. To identify the relationships between muscle function, miRNA signaling pathways, the presence of vascular calcifications and the severity of cardiovascular comorbidities, we initially enrolled 90 subjects on hemodialysis therapy and collected complete data from 46 subjects. A group of 26 subjects inactiv group (INC) was monitored during 12 weeks of physical inactivity and another group of 20 patients exercise group (EXC) was followed during 12 weeks of intradialytic, moderate intensity, resistance training intervention applied three times per week. In both groups, we assessed the expression levels of myo-miRNAs, proteins, and muscle function (MF) before and after the 12-week period. Data on the presence of vascular calcifications and the severity of cardiac comorbidities were collected from the patients' EuCliD records. Using a full structural equitation modelling of the total study sample, we found that the higher the increase in MF was observed in patients, the higher the probability of a decrease in the expression of miR-206 and TRIM63 and the lower severity of cardiac comorbidities. A reduced structural model in INC patients showed that the higher the decrease in MF, the higher the probability of the presence of calcifications and the higher severity of cardiac comorbidities. In EXC patients, we found that the higher the increase in MF, the lower the probability of higher severity of cardiovascular comorbidities.
心血管合并症是透析患者死亡的独立危险因素。miRNA 信号在血管老化和心脏健康中起着重要作用，而体力活动是透析患者心血管合并症的主要非药物治疗方法。为了确定肌肉功能、miRNA 信号通路、血管钙化的存在与心血管合并症的严重程度之间的关系，我们最初招募了 90 名接受血液透析治疗的患者，并从 46 名患者中收集了完整的数据。一组 26 名不活动组（INC）在 12 周的非体力活动期间进行监测，另一组 20 名运动组（EXC）在 12 周的血液透析期间进行监测，每周进行三次中等强度的抗阻训练。在这两组患者中，我们在 12 周的治疗前后评估了肌源性 miRNA、蛋白质和肌肉功能（MF）的表达水平。血管钙化的存在和心脏合并症的严重程度的数据从患者的 EuCliD 记录中收集。使用总研究样本的全结构等距模型，我们发现患者的 MF 增加越多，miR-206 和 TRIM63 的表达降低的概率越高，心脏合并症的严重程度越低。在 INC 患者中，简化的结构模型显示 MF 降低越多，钙化的存在和心脏合并症的严重程度越高的概率越大。在 EXC 患者中，我们发现 MF 增加越多，心血管合并症严重程度越高的概率越低。
