Department of Thoracic Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, Shandong, People's Republic of China.
Department of Cardio-Thoracic Surgery, The People's Hospital of Hai Yang City, Yantai, 264000, Shandong, People's Republic of China.
Exp Cell Res. 2021 Aug 1;405(1):112661. doi: 10.1016/j.yexcr.2021.112661. Epub 2021 May 25.
Twist related protein 2 (TWIST2) plays an important role in bone development, tumorigenesis, tumour progression and epithelial mesenchymal transition (EMT). At present, there are few reports about the role of TWIST2 in lung cancer, which need to be further explored. Therefore, the purpose of this study is to explore the role and molecular mechanism of TWIST2 in the occurrence and development of lung cancer. The expression of TWIST2 in tissues of patients and cell lines was measured using RT-qPCR and western blotting. MTT and CCK8 assays were used to detect cell proliferation and viability. Western blotting was used to measure the expression of EMT-related proteins, including E-cadherin, N-cadherin, Vimentin and Slug. The results revealed that TWIST2 is lowly expressed in the tissues of lung cancer patients and cell lines. Further studies found that overexpression of TWIST2 significantly induced apoptosis and promoted the expression of E-cadherin, as well as inhibiting the expression of N-cadherin, Vimentin and Slug. More importantly, TWIST2 induced oxidative stress in lung cancer cells. In addition, TWIST2 regulated the FGF21 and AMPK/mTOR signalling pathway, which is involved in the molecular mechanism of the gene in lung cancer cells. We suggest that the mechanism of TWIST2 inhibition of the progression of lung cancer is by regulating the FGF21-mediated AMPK/mTOR signalling pathway.
卷曲相关蛋白 2（TWIST2）在骨骼发育、肿瘤发生、肿瘤进展和上皮间质转化（EMT）中发挥重要作用。目前，关于 TWIST2 在肺癌中的作用的报道较少，需要进一步探索。因此，本研究旨在探讨 TWIST2 在肺癌发生发展中的作用及其分子机制。采用 RT-qPCR 和 Western blot 检测患者组织和细胞系中 TWIST2 的表达。MTT 和 CCK8 检测细胞增殖和活力。Western blot 检测 EMT 相关蛋白（包括 E-cadherin、N-cadherin、Vimentin 和 Slug）的表达。结果表明，TWIST2 在肺癌患者组织和细胞系中低表达。进一步研究发现，TWIST2 的过表达显著诱导细胞凋亡，促进 E-cadherin 的表达，同时抑制 N-cadherin、Vimentin 和 Slug 的表达。更重要的是，TWIST2 在肺癌细胞中诱导氧化应激。此外，TWIST2 调节 FGF21 和 AMPK/mTOR 信号通路，这涉及到该基因在肺癌细胞中的分子机制。我们认为 TWIST2 抑制肺癌进展的机制是通过调节 FGF21 介导的 AMPK/mTOR 信号通路。
