Department of Environmental Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Sustainable Environment Research Center, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2021 Sep;336:125313. doi: 10.1016/j.biortech.2021.125313. Epub 2021 May 21.
Thermosynechococcus sp. CL-1 (TCL-1), a thermophilic cyanobacterium from a hot spring in Taiwan, has been known of its efficiency in CO fixation, byproducts production (pigments, macromolecules). This study observed the performance of TCL-1 in CO fixation, estrogen degradation, and monosaccharide production under various levels of Dissolved Inorganic Nitrogen (DIN) and 17β-estradiol (E2) as nitrogen supply and estrogen addition. Under nitrogen starvation, TCL-1 performed similar results on CO fixation rate and biomass production but enhanced the monosaccharide production compared to the cases of high nitrogen supply. The highest CO fixation rate and glucose productivity reached to 151.8 ± 6.6 and 38.1 ± 0.9 mg/L/h, under DIN level of 0.58 mM and 0.5 mg/L E2. Adding E2 in the system did not inhibit the performance of TCL-1. During the cultivation, TCL-1 converted E2 into E1 and the biodegradation was the main path for estrogen degradation. Total E2 degradation reached to 69.4 ± 2.0%.
热生栖热菌（Thermosynechococcus sp. CL-1，TCL-1）是一种来自台湾温泉的嗜热蓝藻，以其高效的 CO 固定和副产物（色素、生物大分子）生产能力而闻名。本研究观察了 TCL-1 在不同溶解无机氮（DIN）和 17β-雌二醇（E2）水平下作为氮源和雌激素添加物对 CO 固定、雌激素降解和单糖生产的性能。在氮饥饿条件下，TCL-1 在 CO 固定率和生物量生产方面表现出相似的结果，但与高氮供应相比，单糖产量有所提高。在 DIN 水平为 0.58 mM 和 0.5 mg/L E2 时，最高 CO 固定率和葡萄糖生产力分别达到 151.8±6.6 和 38.1±0.9 mg/L/h。在该系统中添加 E2 不会抑制 TCL-1 的性能。在培养过程中，TCL-1 将 E2 转化为 E1，且雌激素降解的主要途径是生物降解。总 E2 降解率达到 69.4±2.0%。
