Department of Environmental Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Sustainable Environment Research Center, National Cheng Kung University, Tainan 701, Taiwan.
J Biotechnol. 2023 Aug 20;373:1-11. doi: 10.1016/j.jbiotec.2023.06.004. Epub 2023 Jun 15.
Recently, concern on several environmental issues including the pollutant discharge and high concentration of CO have gained high interest due to its impact on ecosystem and global warming effect, respectively. Implementation of photosynthetic microorganism carries out numerous advantages including high efficiency of CO fixation, the great endurance under extreme conditions and generation of valuable bioproducts. Thermosynechococcus sp. CL-1 (TCL-1), a cyanobacterium, has the ability to perform CO fixation and accumulation of various byproducts under extreme conditions like high temperature and alkalinity, presence of estrogen, or even using swine wastewater. This study aimed to assess TCL-1 performance under various endocrine disruptor compounds (bisphenol-A, 17-β-estradiol/E2, and 17-α-ethynilestradiol/EE2), concentrations (0-10 mg/L), light intensities (500-2000 µE/m/s), and dissolved inorganic carbon/DIC levels (0-113.2 mM). Addition of E2 content even until 10 mg/L carried out insignificant biomass growth interruption along with the improvement in CO fixation rate (79.8 ± 0.1 mg/L/h). Besides the influence of E2, application of higher DIC level and light intensity also enhanced the CO fixation rate and biomass growth. The highest biodegradation of E2 at 71% was achieved by TCL-1 in the end of 12 h cultivation period. TCL-1 dominantly produced protein (46.7% ± 0.2%), however, production of lipid and carbohydrate (39.5 ± 1.5 and 23.3 ± 0.9%, respectively) also could be considered as the potential source for biofuel production. Thus, this study can provide an efficient strategy in simultaneously dealing with environmental issues with side advantage in production of macromolecules.
最近，由于其对生态系统的影响和全球变暖效应，人们对包括污染物排放和高浓度 CO 在内的几个环境问题表示关注。光合微生物的实施具有许多优点，包括高效率的 CO 固定、在极端条件下的巨大耐力和有价值的生物制品的产生。嗜热蓝藻 CL-1（TCL-1）是一种蓝藻，能够在高温、高碱性、雌激素存在甚至使用猪废水等极端条件下进行 CO 固定和各种副产物的积累。本研究旨在评估 TCL-1 在各种内分泌干扰化合物（双酚 A、17-β-雌二醇/E2 和 17-α-乙炔雌二醇/EE2）、浓度（0-10mg/L）、光照强度（500-2000µE/m/s）和溶解无机碳/DIC 水平（0-113.2mM）下的性能。即使添加 10mg/L 的 E2 含量，也不会中断生物量的生长，同时还能提高 CO 固定率（79.8±0.1mg/L/h）。除了 E2 的影响外，较高的 DIC 水平和光照强度的应用也能提高 CO 固定率和生物量的生长。在 12 小时培养期结束时，TCL-1 对 E2 的最高生物降解率达到 71%。TCL-1 主要产生蛋白质（46.7%±0.2%），但脂质和碳水化合物的产量（39.5%±1.5%和 23.3%±0.9%）也可被视为生物燃料生产的潜在来源。因此，本研究可为同时处理环境问题提供一种有效的策略，并具有生产大分子的优势。
