Narindri Rara Winayu Birgitta, Hsueh Hsin-Ta, Chu Hsin
Department of Environmental Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Sustainable Environment Research Center, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2022 Nov;364:128105. doi: 10.1016/j.biortech.2022.128105. Epub 2022 Oct 13.
Cultivation of Cyanobacteria is preferable for CO fixation process due to its efficiency and production of beneficial byproducts like phycocyanin. In this study, Thermosynechococcus sp. CL-1 (TCL-1) was cultivated in a 30 L flat panel photobioreactor using a 3-fold-modified Fitzgerald medium with 113.2 mM dissolved inorganic carbon. The highest CO fixation rate of 21.98 ± 1.52 mg/L/h was followed by higher lipid content (49.91 % dry weight content or %dwc) than the generated carbohydrate (24.22 %dwc). TCL-1 also potentially produced phycocyanin that was dominated by C-phycocyanin (98.10 ± 6.67 mg/g) along with a lower amount of allophycocyanin and phycoerythrin under extraction using various types of solvent. Stability of phycocyanin extract was further examined during storage under various temperatures and light illuminations. Extraction with 36 % glucose solvent presented a protective effect to phycocyanin from heat and photo-damage which was proven by the kinetics study of phycocyanin degradation in this study.
由于蓝藻的效率以及诸如藻蓝蛋白等有益副产物的产生，其培养对于二氧化碳固定过程而言是更可取的。在本研究中，嗜热栖热菌CL-1（TCL-1）在30升平板光生物反应器中使用含有113.2毫摩尔溶解无机碳的三倍改良菲茨杰拉德培养基进行培养。最高二氧化碳固定率为21.98±1.52毫克/升/小时，随后其脂质含量（干重含量或%dwc为49.91%）高于所产生的碳水化合物（24.22%dwc）。TCL-1还潜在地产生了藻蓝蛋白，在使用各种类型溶剂进行提取时，其主要为C-藻蓝蛋白（98.10±6.67毫克/克），同时别藻蓝蛋白和藻红蛋白的含量较低。在不同温度和光照条件下储存期间，进一步检测了藻蓝蛋白提取物的稳定性。本研究中藻蓝蛋白降解的动力学研究证明，用36%葡萄糖溶剂提取对藻蓝蛋白具有抗热和抗光损伤的保护作用。
