College of Food Science, National R&D Professional Center for Berry Processing, National Engineering and Technology of Research Center for Small Berry, Key Laborotary of Healthy Food Nutrition and Innovative Manufacturing, Shenyang Agricultural University, Liaoning Province, Shenyang, Liaoning 110866, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
J Agric Food Chem. 2021 Jun 9;69(22):6200-6213. doi: 10.1021/acs.jafc.1c00082. Epub 2021 May 28.
Blueberry anthocyanins are well known for their beneficial biological activities. However, the poor bioavailability of anthocyanins limits their functional capacity . Our current study aimed to detect the effects of α-casein on the absorption of blueberry anthocyanins and their metabolites in rats. Blueberry anthocyanins with and without α-casein were intragastrically administered to two groups of rats and their blood samples were collected within 24 h. Results illustrated that rapid absorption of anthocyanins was observed in the rat plasma, but their concentration was relatively low. With the complexation of α-casein, the maximum concentration () of bioavailable anthocyanins and metabolites could increase by 1.5-10.1 times ( < 0.05 or < 0.01). The promotional effect on the plasma absorption of malvidin-3--galactoside and vanillic acid was outstanding with the increasing from 0.032 to 0.323 and from 0.360 to 1.902 μg/mL, respectively ( < 0.01). Besides, the molecular docking models presented that anthocyanins could enter the structural cavity and interact with amino acid residues of α-casein, which was in accordance with the improved bioavailability of anthocyanins. Therefore, α-casein could assist more blueberry anthocyanins and their metabolites to enter blood circulation.
蓝莓花色苷以其有益的生物活性而闻名。然而，花色苷的生物利用度差限制了其功能能力。本研究旨在检测α-酪蛋白对蓝莓花色苷及其代谢物在大鼠体内吸收的影响。将含或不含α-酪蛋白的蓝莓花色苷经胃内给予两组大鼠，并在 24 h 内采集其血液样本。结果表明，花色苷在大鼠血浆中迅速吸收，但浓度相对较低。与α-酪蛋白络合后，生物利用度花色苷和代谢物的最大浓度()可增加 1.5-10.1 倍(<0.05 或<0.01)。花色苷-3-O-半乳糖苷和香草酸的血浆吸收促进作用显著，分别从 0.032 μg/mL 增加到 0.323 μg/mL 和从 0.360 μg/mL 增加到 1.902 μg/mL(<0.01)。此外，分子对接模型表明，花色苷可以进入结构腔并与α-酪蛋白的氨基酸残基相互作用，这与花色苷生物利用度的提高是一致的。因此，α-酪蛋白可以帮助更多的蓝莓花色苷及其代谢物进入血液循环。
