口腔扁平苔藓中环状 RNA 的表达谱。

Expression profile of circular RNAs in oral lichen planus.

机构信息

Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; National Clinical Research Center for Oral Diseases, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.

出版信息

Ann Palliat Med. 2021 May;10(5):5205-5217. doi: 10.21037/apm-20-2253. Epub 2021 May 14.

DOI:10.21037/apm-20-2253

PMID:34044570

Abstract

BACKGROUND

This study sought to identify the circular RNAs (circRNAs) differentially expressed in oral lichen planus (OLP) to investigate the possible role of circRNAs in this disease's pathogenesis.

METHODS

Six OLP and six normal oral mucosal tissues were used for circRNA detection and sequencing. 10 selected circRNAs were verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A gene ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to predict the functions of circRNAs in OLP. TargetScan and miRanda were applied to predict targeted micro (mi)RNAs and messenger (m)RNAs of circRNAs, and competing endogenous (ce)RNA networks were mapped.

RESULTS

One hundred and thirty-five circRNAs were identified differentially expressed in OLP tissues compared to normal control tissues, including 83 upregulated circRNAs, and 52 down-regulated circRNAs. RT-qPCR confirmed that 10 circRNAs were all abnormally expressed in OLP. The GO functional analysis and KEGG pathway analysis showed that differentially expressed circRNAs were involved in 535 GO functional items and 78 signal pathways. A ceRNA network analysis showed that circRNAs might interact with a variety of miRNAs.

CONCLUSIONS

This study mapped the expression profile of abnormally expressed circRNAs in OLP tissues for the first time and showed that circRNAs appear to play an important role in the pathogenesis of OLP.

摘要

背景

本研究旨在鉴定口腔扁平苔藓（OLP）中差异表达的环状 RNA（circRNAs），以探讨 circRNAs 在该疾病发病机制中的可能作用。

方法

使用 6 例 OLP 和 6 例正常口腔黏膜组织进行 circRNA 检测和测序。通过逆转录定量聚合酶链反应（RT-qPCR）验证了 10 个选定的 circRNAs。进行基因本体论（GO）功能分析和京都基因与基因组百科全书（KEGG）途径分析，以预测 circRNAs 在 OLP 中的功能。应用 TargetScan 和 miRanda 预测 circRNAs 的靶向微小 RNA（miRNA）和信使 RNA（mRNA），并绘制竞争内源 RNA（ceRNA）网络。

结果

与正常对照组织相比，在 OLP 组织中鉴定出 135 个差异表达的 circRNAs，包括 83 个上调的 circRNAs 和 52 个下调的 circRNAs。RT-qPCR 证实 10 个 circRNAs 在 OLP 中均异常表达。GO 功能分析和 KEGG 途径分析表明，差异表达的 circRNAs 涉及 535 个 GO 功能条目和 78 个信号通路。ceRNA 网络分析表明，circRNAs 可能与多种 miRNA 相互作用。