Wang Hao-Yu, Shen Xue-Min, Shen Zheng-Yu
Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Oct;31(5):535-539.
To construct the expression profile of circular RNA (circRNA) in human oral lichen planus (OLP), and to identify and validate the differentially expressed circRNA in oral lichen planus tissues and provide theoretical basis for the diagnosis and treatment of this disease.
Six patients newly diagnosed with OLP from September to December 2018 in the Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital and 6 healthy volunteers were enrolled in this study. RNA sequencing and evaluation in OLP tissues and normal oral mucosa were performed by high-throughput RNA sequencing technology, and the differences between groups were analyzed. qRT-PCR was used to validate the results. Statistical analysis was conducted with SPSS 24.0 software package. Finally, bioinformatics techniques GO (Gene Ontology) enrichment analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway were used to analyze the functions and related pathways of the dysregulated genes.
According to the sequencing results, 85 differentially expressed circRNAs with fold change > 2 were identified in OLP tissues compared to the normal oral mucosa, including 66 upregulated circRNAs and 19 downregulated circRNAs. Three circRNAs with the most significant up-regulation and down-regulation were selected for qRT-PCR verification in expanded samples, and the results were consistent with the sequencing results. Bioinformatics analysis suggested that the differentially expressed circRNAs may play an important role in the occurrence and progression of oral lichen planus.
Differentially expressed circRNAs between oral lichen planus tissues and normal oral mucosa were identified, which may be involved in the pathogenic mechanism of oral lichen planus and could be potential biomarkers for diagnosis and treatment of this disease.
构建人口腔扁平苔藓（OLP）中环状RNA（circRNA）的表达谱，鉴定并验证口腔扁平苔藓组织中差异表达的circRNA，为该疾病的诊断和治疗提供理论依据。
选取2018年9月至12月在上海第九人民医院口腔黏膜病科新诊断的6例OLP患者和6名健康志愿者作为研究对象。采用高通量RNA测序技术对OLP组织和正常口腔黏膜进行RNA测序及评估，并分析组间差异。采用qRT-PCR验证结果。使用SPSS 24.0软件包进行统计分析。最后，运用生物信息学技术基因本体论（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析来分析失调基因的功能及相关通路。
根据测序结果，与正常口腔黏膜相比，在OLP组织中鉴定出85个差异表达的circRNA，其变化倍数>2，其中66个circRNA上调，19个circRNA下调。选择上调和下调最显著的3个circRNA在扩大样本中进行qRT-PCR验证，结果与测序结果一致。生物信息学分析表明，差异表达的circRNA可能在口腔扁平苔藓的发生和发展中起重要作用。
鉴定出了口腔扁平苔藓组织与正常口腔黏膜之间差异表达的circRNA，它们可能参与口腔扁平苔藓的致病机制，并且可能成为该疾病诊断和治疗的潜在生物标志物。
