Solano Angela Giraldo, Dupuy Joan, Therriault Hélène, Liberelle Benoît, Faucheux Nathalie, Lauzon Marc-Antoine, Virgilio Nick, Paquette Benoit
Center for Research in Radiotherapy, Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada.
Centre de recherche sur les systèmes polymères et composites à haute performance (CREPEC), Department of Chemical Engineering, Polytechnique Montréal, 2900, boul. Édouard-Montpetit, Montréal, Québec, Canada.
Carbohydr Polym. 2021 Aug 15;266:118115. doi: 10.1016/j.carbpol.2021.118115. Epub 2021 Apr 24.
To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely. Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap. Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm. The F98 GBM cells migrated in the pores and mainly accumulated in the center of the matrix. Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix. Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.
为了克服浸润在大脑中的胶质母细胞瘤（GBM）细胞的辐射抗性，我们提议将这些癌细胞吸引到一个能够安全地给予致死辐射剂量的陷阱中。在此，我们制备并表征了一种基于海藻酸钠的大孔水凝胶作为潜在的癌细胞陷阱。微计算机X射线断层扫描显示，水凝胶基质包含平均直径为300μm的相互连通的孔隙。F98 GBM细胞在孔隙中迁移并主要聚集在基质中心。根据添加的癌细胞数量，将RGD细胞粘附肽接枝到海藻酸盐上导致保留在基质中的F98细胞（其过表达相关的αvβ3和αvβ5结合整合素）数量增加4至10倍。最后，25 Gy的辐射剂量消除了捕获在基质中的所有F98细胞，而没有显著改变基质的机械性能。
