Tanaka T, Kita H
J Biochem. 1977 Dec;82(6):1689-93. doi: 10.1093/oxfordjournals.jbchem.a131865.
The change of the mating factor activity during the culture of Saccharomyces cerevisiae X-2180 1B, an alpha-mating type haploid strain, were followed. The activity increased rapidly during the exponential phase of growth, reached a maximum during the early stationary phase and then decreased. Oligopeptides comprising partial sequences of the mating factor were isolated from the culture fluids at various phases of cell growth. We concluded that the mating factor, a tridecapeptide, was degraded during culture into two peptides, Trp-His-Trp-Leu-Gln-Leu and Lys-Pro-Gly-Gln-Pro-Met-Tyr, by cleavage of the peptide bond between Leu-6 and Lys-7 of the mating factor. A dodecapeptide lacking the N-terminal Trp residue was not detected at any stage of cell growth examined.
对酿酒酵母X-2180 1B（一种α-交配型单倍体菌株）培养过程中交配因子活性的变化进行了跟踪。在生长指数期，活性迅速增加，在稳定期早期达到最大值，然后下降。在细胞生长的各个阶段，从培养液中分离出包含交配因子部分序列的寡肽。我们得出结论，交配因子（一种十三肽）在培养过程中通过切断交配因子Leu-6和Lys-7之间的肽键，降解为两种肽，即Trp-His-Trp-Leu-Gln-Leu和Lys-Pro-Gly-Gln-Pro-Met-Tyr。在所检测的细胞生长的任何阶段都未检测到缺少N端Trp残基的十二肽。
