State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Mediators Inflamm. 2021 May 10;2021:6611219. doi: 10.1155/2021/6611219. eCollection 2021.
(L.) Britton is a classic herbal plant used widely against asthma in China. But its mechanism of beneficial effect remains undermined. In the study, the antiallergic asthma effects of Perilla leaf extract (PLE) were investigated, and the underlying mechanism was also explored. Results showed that PLE treatment significantly attenuated airway inflammation in OVA-induced asthma mice, by ameliorating lung pathological changes, inhibiting recruitment of inflammatory cells in lung tissues and bronchoalveolar lavage fluid (BALF), decreasing the production of inflammatory cytokines in the BALF, and reducing the level of immunoglobulin in serum. PLE treatment suppressed inflammatory response in antigen-induced rat basophilic leukemia 2H3 (RBL-2H3) cells as well as in OVA-induced human peripheral blood mononuclear cells (PBMCs). Furthermore, PLE markedly inhibited the expression and phosphorylation of Syk, NF-B, PKC, and cPLA both and . By cotreating with inhibitors (BAY61-3606, Rottlerin, BAY11-7082, and arachidonyl trifluoromethyl ketone) , results revealed that PLE's antiallergic inflammatory effects were associated with the inhibition of Syk and its downstream signals NF-B, PKC, and cPLA. Collectively, the present results suggested that PLE could attenuate allergic inflammation, and its mechanism might be partly mediated through inhibiting the Syk pathway.
（L.）荆芥是一种经典的草药植物，在中国被广泛用于治疗哮喘。但其有益作用的机制仍未得到证实。本研究旨在探讨紫苏叶提取物（PLE）的抗过敏性哮喘作用及其潜在机制。结果表明，PLE 治疗可显著减轻 OVA 诱导的哮喘小鼠的气道炎症，改善肺部病理变化，抑制肺组织和支气管肺泡灌洗液（BALF）中炎症细胞的募集，减少 BALF 中炎症细胞因子的产生，并降低血清中免疫球蛋白的水平。PLE 处理还可抑制抗原诱导的大鼠嗜碱性白血病 2H3（RBL-2H3）细胞以及 OVA 诱导的人外周血单核细胞（PBMCs）中的炎症反应。此外，PLE 可显著抑制 Syk、NF-B、PKC 和 cPLA 的表达和磷酸化。通过与抑制剂（BAY61-3606、Rottlerin、BAY11-7082 和 arachidonyl trifluoromethyl ketone）共同处理，结果表明 PLE 的抗过敏炎症作用与抑制 Syk 及其下游信号 NF-B、PKC 和 cPLA 有关。综上所述，本研究结果表明，PLE 可减轻过敏性炎症，其机制可能部分通过抑制 Syk 途径介导。
