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1
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Mediators Inflamm. 2021 May 10;2021:6611219. doi: 10.1155/2021/6611219. eCollection 2021.
2
Antiallergic activities of shallot (Allium ascalonicum L.) and its therapeutic effects in allergic rhinitis.洋葱（Allium ascalonicum L.）的抗过敏活性及其在过敏性鼻炎中的治疗效果。
Asian Pac J Allergy Immunol. 2022 Dec;40(4):393-400. doi: 10.12932/AP-300319-0529.
3
Inhibitory effect of aqueous extract of Cuminum cyminum L. seed on degranulation of RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice.孜然种子水提取物对RBL-2H3细胞脱颗粒及小鼠被动皮肤过敏反应的抑制作用。
Cytotechnology. 2019 Apr;71(2):599-609. doi: 10.1007/s10616-019-00309-2. Epub 2019 Mar 23.
4
Biological and epidemiological evidence of anti-allergic effects of traditional Japanese food ume (Prunus mume).传统日本食物梅（Prunus mume）具有抗过敏作用的生物学和流行病学证据。
Sci Rep. 2018 Aug 3;8(1):11638. doi: 10.1038/s41598-018-30086-5.
5
Cirsium maritimum Makino Inhibits the Antigen/Immunoglobulin-E-Mediated Allergic Response In Vitro and In Vivo.滨海蓟抑制体外和体内抗原/免疫球蛋白E介导的过敏反应。
J Agric Food Chem. 2017 Sep 27;65(38):8386-8391. doi: 10.1021/acs.jafc.7b03322. Epub 2017 Sep 19.
6
Anti-allergic effects of His-Ala-Gln tripeptide in vitro and in vivo.
Biosci Biotechnol Biochem. 2017 Feb;81(2):380-383. doi: 10.1080/09168451.2016.1243984. Epub 2016 Oct 12.
7
Synergistic antitumor effect of a combination of paclitaxel and carboplatin with nobiletin from Citrus depressa on non-small-cell lung cancer cell lines.紫杉醇和卡铂与来自温州蜜柑的川陈皮素联合使用对非小细胞肺癌细胞系的协同抗肿瘤作用。
Planta Med. 2014 Apr;80(6):452-7. doi: 10.1055/s-0034-1368321. Epub 2014 Mar 31.
8
Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells.菠菜水提物对 RBL-2H3 细胞脱颗粒的抑制作用。
Food Chem. 2013 Jan 15;136(2):322-7. doi: 10.1016/j.foodchem.2012.08.079. Epub 2012 Sep 8.
9
New developments in FcepsilonRI regulation, function and inhibition.FcεRI调节、功能及抑制方面的新进展。
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10
Fc{epsilon}RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane.FcεRI介导的肥大细胞脱颗粒需要颗粒不依赖钙而依赖微管向质膜的转运。
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橙皮素和乳铁蛋白联合处理可协同抑制RBL-2H3大鼠嗜碱性白血病细胞的脱颗粒。

Degranulation of RBL-2H3 rat basophilic leukemia cells is synergistically inhibited by combined treatment with nobiletin and lactoferrin.

作者信息

Miyake Kaho, Tanaka Mamoru, Yokoyama Sayaka, Rui Lu, Koida Ayaka, Kozai Hana, Okamoto Takeaki

机构信息

Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501 Japan.

Department of Food and Nutritional Environment, Kinjo Gakuin University, Nagoya, Aichi Japan.

出版信息

Cytotechnology. 2024 Jun;76(3):341-349. doi: 10.1007/s10616-024-00625-2. Epub 2024 Apr 4.

DOI:10.1007/s10616-024-00625-2
PMID:38736731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11082116/
Abstract

The aim of this study was to elucidate the anti-allergic effects of polymethoxyflavonoids in combination with milk proteins and the mechanism of inhibition. Three polymethoxyflavonoids and two milk proteins were exposed to the rat basophilic leukemia cell line RBL-2H3. β-hexosaminidase was used as an indicator of degranulation inhibition. The mechanism of inhibition was examined by measuring intracellular Ca levels and western blot method. In the degranulation inhibition test with polymethoxyflavonoids and milk proteins alone, nobiletin was the strongest inhibitor in the polymethoxyflavonoid group and lactoferrin in the milk protein group. Next, co-stimulation with nobiletin and lactoferrin showed stronger synergistic degranulation inhibition than treatment with nobiletin or lactoferrin alone. Western blot analysis showed that co-stimulation with nobiletin and lactoferrin significantly downregulated the induction of phospholipase Cγ 1 phosphorylation. The degranulation response in RBL-2H3 cells was synergistically suppressed by co-stimulation of nobiletin and lactoferrin acting on both Ca-dependent and Ca-independent pathways.

摘要

本研究的目的是阐明聚甲氧基黄酮与乳蛋白联合使用的抗过敏作用及其抑制机制。将三种聚甲氧基黄酮和两种乳蛋白作用于大鼠嗜碱性白血病细胞系RBL-2H3。采用β-己糖胺酶作为脱颗粒抑制的指标。通过测量细胞内钙水平和蛋白质免疫印迹法检测抑制机制。在单独使用聚甲氧基黄酮和乳蛋白的脱颗粒抑制试验中，川陈皮素是聚甲氧基黄酮组中最强的抑制剂，乳铁蛋白是乳蛋白组中最强的抑制剂。接下来，川陈皮素和乳铁蛋白共同刺激显示出比单独使用川陈皮素或乳铁蛋白更强的协同脱颗粒抑制作用。蛋白质免疫印迹分析表明，川陈皮素和乳铁蛋白共同刺激显著下调了磷脂酶Cγ1磷酸化的诱导。川陈皮素和乳铁蛋白共同作用于钙依赖性和非钙依赖性途径，协同抑制了RBL-2H3细胞的脱颗粒反应。