Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, PR China.
J Anat. 2021 Oct;239(4):913-919. doi: 10.1111/joa.13461. Epub 2021 May 28.
Adult neurons of several reptiles still retain the ability of axonal regeneration in contrast to the low intrinsic regenerative capacity of those in the central nervous system (CNS) in mammals. This feature of the reptilian neurons has provided a perfect model for elucidating the regenerative mechanism lost in the mammalian counterparts. However, little information is available on the primary culture method of adult reptilian neurons, which greatly limits their valuable applications. In the present study, we introduced a simple and easy method for the isolation, culture, and identification of neurons from the cerebral cortex using the adult geckos. The cultured cells were further identified by immunofluorescence using antibodies against neuron-specific markers β-Ⅲ-tubulin and NeuN. The cortical neurons from adult gecko displayed spindle-shaped, bipolar, or multipolar morphology with a plump soma. This primary culture method for adult reptilian neurons will be beneficial for comparative studies of neuronal biology in various vertebrates.
与哺乳动物中枢神经系统（CNS）中神经元内在再生能力低相比，几种爬行动物的成年神经元仍保留有轴突再生的能力。这种爬行动物神经元的特性为阐明在哺乳动物对应物中丧失的再生机制提供了一个完美的模型。然而，关于成年爬行动物神经元的原代培养方法的信息很少，这极大地限制了它们的有价值的应用。在本研究中，我们使用成年壁虎介绍了一种从大脑皮质分离、培养和鉴定神经元的简单易行的方法。通过使用神经元特异性标志物 β-Ⅲ-微管蛋白和 NeuN 的抗体进行免疫荧光染色，进一步鉴定培养的细胞。来自成年壁虎的皮质神经元呈梭形、双极或多极形态，具有饱满的胞体。这种成年爬行动物神经元的原代培养方法将有助于各种脊椎动物神经元生物学的比较研究。
