Wang Shuo, He Yuqi, Zhang Huiqian, Chen Li, Cao Liang, Yang Leiluo, Wang Chunqing, Pan Yujie, Tang Qian, Tan Wei, Dou Xiaowei, Li Qing
Department of Orthopedics Traumatic, The Affiliated Hospital of Guizhou Medical University, Guiyang City, China.
School of Clinical Medicine, Guizhou Medical University, Guiyang, China.
Front Cell Neurosci. 2021 Mar 15;15:630882. doi: 10.3389/fncel.2021.630882. eCollection 2021.
Cerebrospinal fluid-touching neurons (CSF-cNs) exist in the region surrounding the central canal of the spinal cord, which locate in the adult neurogenic niche. Previous research showed that CSF-cNs expressed the molecular markers of immature neural cells . Here, we explored the potential of CSF-cNs as neural stem cell . We first found that PKD2L1 CSF-cNs, isolating by FACS using the molecular marker PKD2L1 of CSF-cNs, expressed neural stem cells markers like Nestin, Sox2, and GFAP by immunofluorescence staining. PKD2L1 CSF-cNs were able to form neurospheres and passaged . Immunofluorescence staining showed that the neurospheres forming by PKD2L1 CSF-cNs also expressed neural stem cell markers Nestin, Sox2 and GFAP. The neurospheres expressed proliferation markers Ki67 and PCNA by immunofluorescence staining, indicating that the neurospheres forming by PKD2L1 CSF-cNs were proliferative. The neurospheres, forming by CSF-cNs, had the ability of differentiation into neurons, astrocytes, and oligodendrocytes. Collectively, our data suggested that PKD2L1 CSF-cNs have the properties of neural stem cells and may provide a promising approach for the repair of spinal cord injury.
脑脊液接触神经元（CSF-cNs）存在于脊髓中央管周围区域，该区域位于成体神经发生微环境中。先前的研究表明，CSF-cNs表达未成熟神经细胞的分子标志物。在此，我们探讨了CSF-cNs作为神经干细胞的潜力。我们首先发现，通过使用CSF-cNs的分子标志物PKD2L1经荧光激活细胞分选（FACS）分离得到的PKD2L1 CSF-cNs，通过免疫荧光染色表达神经干细胞标志物，如巢蛋白（Nestin）、性别决定区Y盒2（Sox2）和胶质纤维酸性蛋白（GFAP）。PKD2L1 CSF-cNs能够形成神经球并传代。免疫荧光染色显示，由PKD2L1 CSF-cNs形成的神经球也表达神经干细胞标志物Nestin、Sox2和GFAP。通过免疫荧光染色，神经球表达增殖标志物Ki67和增殖细胞核抗原（PCNA），这表明由PKD2L1 CSF-cNs形成的神经球具有增殖能力。由CSF-cNs形成的神经球具有分化为神经元、星形胶质细胞和少突胶质细胞的能力。总的来说，我们的数据表明PKD2L1 CSF-cNs具有神经干细胞的特性，可能为脊髓损伤的修复提供一种有前景的方法。
