Tan Longfei, Liu Tianlong, Fu Changhui, Wang Shengping, Fu Shiyan, Ren Jun, Meng Xianwei
Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
J Mater Chem B. 2016 Feb 7;4(5):859-866. doi: 10.1039/c5tb02205c. Epub 2016 Jan 11.
With the aim of fabricating multifunctional nanoplatforms for cancer diagnosis and therapy, we design hollow ZrO nanospheres as novel nanocarriers with enhanced CT imaging properties to integrate NIR light absorbing agents and anti-cancer drugs into one nanosystem. Polypyrrole (PPy) is encapsulated into the hollow ZrO nanospheres to endow the nanosystem with strong photothermal transfer capability via an oxidative polymerization method. The anti-cancer drug, doxorubicin (DOX), was loaded into the hollow ZrO nanospheres with a high loading content and Dox could be released from the carrier by NIR photothermal stimulus. The hollow ZrO nanoplatform has been successfully demonstrated for synergistic photothermal-chemo therapy, both in vitro and in vivo. Due to the excellent X-ray attenuation ability of Zr, the biodistribution of this nanosystem was real-time monitored via CT imaging technology using mini swine as animal models. ICP-OES analysis of tissues obtained from the biopsy also confirms the biodistribution. The in vitro and in vivo toxicity evaluation shows that this nanosystem has good biocompatibility and has great prospects for imaging-guided photothermal-chemo cancer therapy.
为了制备用于癌症诊断和治疗的多功能纳米平台，我们设计了中空ZrO纳米球作为具有增强CT成像特性的新型纳米载体，将近红外光吸收剂和抗癌药物整合到一个纳米系统中。通过氧化聚合法将聚吡咯（PPy）封装到中空ZrO纳米球中，赋予纳米系统强大的光热转换能力。将抗癌药物阿霉素（DOX）以高负载量载入中空ZrO纳米球，DOX可通过近红外光热刺激从载体中释放。中空ZrO纳米平台已成功在体外和体内证明了其协同光热化疗作用。由于Zr具有出色的X射线衰减能力，以小型猪为动物模型，通过CT成像技术对该纳米系统的生物分布进行了实时监测。对活检获得的组织进行的ICP-OES分析也证实了生物分布情况。体外和体内毒性评估表明，该纳米系统具有良好的生物相容性，在成像引导的光热化疗癌症治疗方面具有广阔前景。
