Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of Applied Physics, Faculty of Engineering, Rajamangala University of Technology Isan, Khon Kaen Campus, Khon Kaen, 40000, Thailand.
Mikrochim Acta. 2021 May 28;188(6):208. doi: 10.1007/s00604-021-04869-z.
A highly sensitive and novel electrochemical sensor for ciprofloxacin (CIP) has been developed using gold nanoparticles deposited with waste coffee ground activated carbon on glassy carbon electrode (AuNPs/AC/GCE) and combined with supramolecular solvent (SUPRAS). The fabricated AuNPs/AC/GCE displayed good electrocatalytic activity for AuNPs. The addition of SUPRAS, prepared from cationic surfactants namely didodecyldimethylammonium bromide (DDAB) and dodecyltrimethylammonium bromide (DTAB), increased the electrochemical response of AuNPs. The detection of CIP was based on the decrease of the cathodic current of AuNPs. The electrochemical behavior of the modified electrode was investigated using cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. Under optimum conditions, the calibration plot of CIP exhibited a linear response in the range 0.5-25 nM with a detection limit of 0.20 nM. The fabricated electrochemical sensor was successfully applied to determine CIP in milk samples with achieved recoveries of 78.6-110.2% and relative standard deviations of <8.4%. The developed method was also applied to the analysis of pharmaceutical formulation and the results were compared with high-performance liquid chromatography.Graphical abstract.
一种基于金纳米粒子修饰的废咖啡渣活性炭/玻碳电极（AuNPs/AC/GCE）和超分子溶剂（SUPRAS）的新型高灵敏电化学传感器已被开发出来，用于检测环丙沙星（CIP）。所制备的 AuNPs/AC/GCE 对 AuNPs 表现出良好的电催化活性。超分子溶剂（SUPRAS）的加入，由阳离子表面活性剂二癸基二甲基溴化铵（DDAB）和十二烷基三甲基溴化铵（DTAB）制备，增加了 AuNPs 的电化学响应。CIP 的检测是基于 AuNPs 阴极电流的降低。通过循环伏安法、差分脉冲伏安法和电化学阻抗谱研究了修饰电极的电化学行为。在最佳条件下，CIP 的校准曲线在 0.5-25 nM 范围内呈线性响应，检测限为 0.20 nM。该制备的电化学传感器成功地应用于牛奶样品中 CIP 的测定，回收率为 78.6-110.2%，相对标准偏差小于 8.4%。该方法还用于药物制剂的分析，结果与高效液相色谱法进行了比较。
