Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA.
Methods Mol Biol. 2021;2321:75-100. doi: 10.1007/978-1-0716-1488-4_8.
Sepsis results from the dysregulated immune response to infection. While the stimulator and progression of the septic response is poorly understood, the systemic production of a storm of cytokines is common in all etiologies of sepsis. While the complexity of this uncontrolled cascade is difficult to replicate using single molecule agonist, for example, lipopolysaccharide (LPS), several whole organism models can stimulate this cytokine storm. Herein, we detail protocols developed to trigger and analyze the systemic septic response in mouse models using the bacterium Francisella tularensis.
脓毒症是由感染引起的免疫失调反应导致的。尽管脓毒症反应的刺激和进展机制尚未完全阐明，但在所有脓毒症病因中，细胞因子风暴的全身产生是很常见的。虽然使用单个分子激动剂（例如脂多糖（LPS））很难复制这种失控级联反应的复杂性，但有几种全生物体模型可以刺激这种细胞因子风暴。本文详细介绍了使用土拉弗朗西斯菌在小鼠模型中引发和分析全身性脓毒症反应的方法。
