坏死性小肠结肠炎小鼠模型。
A Mouse Model of Necrotizing Enterocolitis.
机构信息
Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO, USA.
出版信息
Methods Mol Biol. 2021;2321:101-110. doi: 10.1007/978-1-0716-1488-4_9.
Abstract
Necrotizing enterocolitis (NEC) is an acute inflammatory disease that unforeseeably develops in very low birth weight premature infants. NEC is characterized by impairment of the intestinal barrier resulting in intestinal necrosis and multisystem organ failure. Animal models of NEC have contributed significantly to a better understanding of the underlying molecular mechanisms of the disease and facilitated the exploration of potential new therapeutic strategies. Here, we provide a detailed protocol that recapitulates some of the main histological and transcriptional features of human NEC in newborn mice.
摘要
坏死性小肠结肠炎（NEC）是一种在极低出生体重早产儿中不可预测发生的急性炎症性疾病。NEC 的特征是肠道屏障受损，导致肠坏死和多器官功能衰竭。NEC 的动物模型极大地促进了对疾病潜在分子机制的更好理解，并为探索潜在的新治疗策略提供了便利。在这里，我们提供了一个详细的方案，该方案再现了新生小鼠中一些与人 NEC 相关的主要组织学和转录特征。
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本文引用的文献
1
Animal models of necrotizing enterocolitis: review of the literature and state of the art.坏死性小肠结肠炎的动物模型：文献综述与最新进展
Innov Surg Sci. 2018 Mar 10;3(2):87-92. doi: 10.1515/iss-2017-0050. eCollection 2018 Jun.
2
Impact of Toll-Like Receptor 4 Signaling in Necrotizing Enterocolitis: The State of the Science.Toll样受体4信号通路在坏死性小肠结肠炎中的作用：科学现状
Clin Perinatol. 2019 Mar;46(1):145-157. doi: 10.1016/j.clp.2018.09.007. Epub 2018 Dec 20.
3
Neonatal intestinal dysbiosis in necrotizing enterocolitis.坏死性小肠结肠炎中的新生儿肠道菌群失调。
Mol Med. 2018 Mar 15;24(1):4. doi: 10.1186/s10020-018-0002-0.
4
Development of the gut microbiota in infancy and its impact on health in later life.婴儿期肠道微生物组的发育及其对生命后期健康的影响。
Allergol Int. 2017 Oct;66(4):515-522. doi: 10.1016/j.alit.2017.07.010. Epub 2017 Aug 18.
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Dextran sodium sulfate (DSS) induces necrotizing enterocolitis-like lesions in neonatal mice.葡聚糖硫酸钠（DSS）可在新生小鼠中诱发坏死性小肠结肠炎样病变。
PLoS One. 2017 Aug 17;12(8):e0182732. doi: 10.1371/journal.pone.0182732. eCollection 2017.
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Br J Nutr. 2016 Oct;116(7):1175-1187. doi: 10.1017/S0007114516002944. Epub 2016 Sep 9.
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