Division of Fundamental AgriScience and Research, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
Biosci Biotechnol Biochem. 2021 Jul 23;85(8):1885-1889. doi: 10.1093/bbb/zbab096.
Young barley leaves (Hordeum vulgare L.) have various health effects and are employed as an ingredient in the production of health-promoting foods. Promoting antiobesity is one such health effect; however, the mechanism and bioactive compounds are unclear. In this research, young barley leaf extract (YB) was demonstrated to possess pancreatic lipase inhibitory activity. The addition of YB to a high-fat diet in mice increased fecal lipid content, indicating reduced absorption of lipids as the mechanism underlying antiobesity effect. The investigation of bioactive compounds in YB resulted in the identification of fructose-bisphosphate aldolase as a proteinous lipase inhibitor. Maximum inhibition of the protein was 45%, but inhibition was displayed at a concentration as low as 16 ng/mL, which is a characteristic inhibition compared with other reported proteinous lipase inhibitors.
嫩麦苗（Hordeum vulgare L.）具有多种保健功效，被用作生产具有保健作用的食品的成分。促进抗肥胖是其具有的一种保健功效，但作用机制和生物活性化合物尚不清楚。本研究表明，嫩麦苗提取物（YB）具有胰脂肪酶抑制活性。YB 添加到高脂饮食的小鼠中可增加粪便脂质含量，表明其抗肥胖作用的机制是减少脂质的吸收。对 YB 中的生物活性化合物进行研究，结果鉴定出了果糖-1,6-二磷酸醛缩酶为蛋白脂肪酶抑制剂。对该蛋白的最大抑制率为 45%，但在低至 16ng/mL 的浓度下即显示出抑制作用，与其他报道的蛋白脂肪酶抑制剂相比，这是一种特征性抑制作用。
