Lee Minhee, Kim Dakyung, Park Mi-Ryeong, Kim Soyoung, Kim Jong-Lae, Kim Ok-Kyung, Lee Jeongmin
Department of Medical Nutrition, Kyung Hee University, Yongin 17104, Korea.
Department of Food Innovation and Health, Kyung Hee University, Yongin 17104, Korea.
Nutr Res Pract. 2024 Oct;18(5):587-601. doi: 10.4162/nrp.2024.18.5.587. Epub 2024 Jun 13.
BACKGROUND/OBJECTIVES: UV radiation is a major factor contributing to DNA damage in skin cells, including stem cells and mesenchymal stem cells, leading to the depletion of these crucial cells. This study examined whether a mixture of Indian gooseberry and barley sprout (IB) could inhibit UVB irradiation and 3-isobutyl-1-methylxanthine (IBMX)-induced photoaging and oxidative stress in the skin using HaCaT, Hs27, and B16F10 cells.
MATERIALS/METHODS: The moisturizing-related factors, the collagen synthesis-related c-Jun N-terminal kinase (JNK)/c-Fos/c-Jun/matrix metalloproteinases (MMPs) pathway, and the melanogenesis-related cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP-responsive binding protein (CREB)/melanocyte inducing transcription factor (MITF)/tyrosinase-related protein (TRP)/tyrosinase activation pathways were analyzed by an enzyme-linked immunosorbent assay, real-time polymerase chain reaction, and Western blot analysis.
The IB complex increased the hyaluronic acid and sphingomyelin levels and the collagenase inhibitory activity, enhanced hydration-related factors, including collagen, (), , () (; ), and (), modulated the inflammatory cytokines levels, antioxidant enzyme activities and the NF-κB/MMPs/cyclooxygenase-2 (COX-2) pathway in UVB-irradiated HaCaT cells, and inhibited wrinkle formation by down-regulation of the JNK/c-Fos/c-Jun/MMP pathway and up-regulation of the ()/small mothers against decapentaplegic homolog (Smad3)/ pathway in UVB-irradiated Hs27 cells. Moreover, the IB complex prevented melanin production by down-regulating the PKA/CREB/MITF/TRP-1/TRP-2 pathway in IBMX-induced B16F10 cells.
These findings suggest that the IB complex has the potential to serve as a safeguard, shielding the skin from UVB radiation-induced photo-damage.
背景/目的：紫外线辐射是导致皮肤细胞（包括干细胞和间充质干细胞）DNA损伤的主要因素，会导致这些关键细胞的损耗。本研究使用HaCaT、Hs27和B16F10细胞，检测印度醋栗和大麦芽混合物（IB）是否能抑制紫外线B（UVB）照射及3 - 异丁基 - 1 - 甲基黄嘌呤（IBMX）诱导的皮肤光老化和氧化应激。
材料/方法：通过酶联免疫吸附测定、实时聚合酶链反应和蛋白质免疫印迹分析，对保湿相关因子、胶原蛋白合成相关的c - Jun氨基末端激酶（JNK）/c - Fos/c - Jun/基质金属蛋白酶（MMPs）通路，以及黑色素生成相关的环磷酸腺苷（cAMP）/蛋白激酶A（PKA）/cAMP反应结合蛋白（CREB）/黑素细胞诱导转录因子（MITF）/酪氨酸酶相关蛋白（TRP）/酪氨酸酶激活通路进行分析。
IB复合物增加了透明质酸和鞘磷脂水平以及胶原酶抑制活性，增强了包括胶原蛋白在内的与水合作用相关的因子（）、（）、（）（；）和（），调节了UVB照射的HaCaT细胞中的炎性细胞因子水平、抗氧化酶活性以及核因子κB（NF - κB）/MMPs/环氧化酶 - 2（COX - 2）通路，并通过下调UVB照射的Hs27细胞中的JNK/c - Fos/c - Jun/MMP通路和上调（）/小的抗五聚体瘫蛋白同源物（Smad3）/通路来抑制皱纹形成。此外，IB复合物通过下调IBMX诱导的B16F10细胞中的PKA/CREB/MITF/TRP - 1/TRP - 2通路来阻止黑色素生成。
这些发现表明，IB复合物有潜力作为一种防护措施，保护皮肤免受UVB辐射诱导的光损伤。
