Department of Clinical Laboratory, Huangzhou District People's Hospital, Huanggang 442000, Hubei, China.
National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
J Stroke Cerebrovasc Dis. 2021 Aug;30(8):105874. doi: 10.1016/j.jstrokecerebrovasdis.2021.105874. Epub 2021 May 25.
Voltage-gated calcium channels (VGCCs) dysfunction is involved in the development of acute ischemic stroke (AIS). As a subunit of VGCC complexes, we detected the levels of α2δ-1 subunit in serum and cerebrospinal fluid (CSF) specimens from AIS patients.
The study included 105 patients with first-ever AIS, who were admitted within 48 hours after stroke onset. The serum and CSF levels of α2δ-1 were measured with ELISA and the severity of AIS patients was evaluated according to the National Institutes of Health Stroke Scale (NIHSS) score. The cerebral infarct volume was calculated through the Pullicino formula based on the cranial CT or MRI scan. C-reactive protein (CRP) and serum amyloid A (SAA) were measured using the latex-enhanced immunoturbidimetric assay.
Compared to the control subjects, the serum α2δ-1 level was significantly increased in AIS patients with large infarct volume and in severe AIS cases with high NIHSS score, which correlated positively with the inflammatory markers CRP and SAA. Furthermore, the concentration of α2δ-1 in CSF was elevated with the infarct volume, which was higher in severe AIS patients.
Our study suggests that the increased α2δ-1 levels in serum and CSF specimens may be used as a potential marker for reflecting VGCCs dysfunction, illness severity and neuroinflammation in AIS patients.
电压门控钙通道（VGCC）功能障碍与急性缺血性脑卒中（AIS）的发展有关。作为 VGCC 复合物的亚基，我们检测了 AIS 患者血清和脑脊液（CSF）标本中α2δ-1 亚基的水平。
本研究纳入了 105 例首次发生的 AIS 患者，这些患者在发病后 48 小时内入院。采用 ELISA 法检测血清和 CSF 中α2δ-1 的水平，并根据美国国立卫生研究院卒中量表（NIHSS）评分评估 AIS 患者的严重程度。根据头颅 CT 或 MRI 扫描，通过 Pullicino 公式计算脑梗死体积。采用胶乳增强免疫比浊法检测 C 反应蛋白（CRP）和血清淀粉样蛋白 A（SAA）。
与对照组相比，大梗死体积的 AIS 患者和 NIHSS 评分较高的重度 AIS 患者的血清α2δ-1 水平显著升高，与炎症标志物 CRP 和 SAA 呈正相关。此外，随着梗死体积的增加，CSF 中α2δ-1 的浓度也升高，在重度 AIS 患者中更高。
我们的研究表明，血清和 CSF 标本中α2δ-1 水平的升高可能可用作反映 AIS 患者 VGCC 功能障碍、疾病严重程度和神经炎症的潜在标志物。
