Chen Zhenzhen, Wang Kaihua, Huang Jianmin, Zheng Guangshan, Lv Yan, Luo Ning, Liang Mingkun, Huang Longjian
Department of Neurology, Guangxi University of Chinese Medicine, Nanning, China.
Department of Neurology, RuiKang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, China.
Cell Physiol Biochem. 2018;45(1):397-405. doi: 10.1159/000486916. Epub 2018 Jan 22.
BACKGROUND/AIMS: Stroke is a major cerebrovascular disease threatening human health and life with high morbidity, disability and mortality. It is aimed to find effective biomarkers for the early diagnosis on stroke.
The expressions of 17 previously reported stroke-associated miRNAs were measured using quantitative RT-PCR and the expressions of plasma high-sensitivity C reactive protein (hs-CRP) and serum interleukin 6 (IL-6), the pro-inflammation markers in brain injury, were examined using enzyme-linked immunosorbent assay in 128 acute ischemic stroke (AIS) patients and control group.
Serum miR-146b expression was significantly increased within 24 hours after stroke onset in patients compared with control group. In addition, the upregulation of serum miR-146b was strong positively correlated with plasma hs-CRP, infarct volume and National Institutes of Health Stroke Scale (NIHSS) score, and moderate positively correlated with serum IL-6 of patients. Importantly, the combination of plasma hs-CRP and serum miR-146b gained a better sensitivity/specificity for prediction of AIS (AUC from 0.782 to 0.863).
Our preliminary findings suggested that upregulated serum miR-146b in acute ischemic stroke might be a potential biomarker for AIS evaluation.
背景/目的：中风是一种威胁人类健康和生命的主要脑血管疾病，具有高发病率、高致残率和高死亡率。旨在寻找用于中风早期诊断的有效生物标志物。
采用定量逆转录聚合酶链反应检测17种先前报道的与中风相关的微小RNA（miRNA）的表达，并采用酶联免疫吸附测定法检测128例急性缺血性中风（AIS）患者和对照组血浆高敏C反应蛋白（hs-CRP）及血清白细胞介素6（IL-6）（脑损伤中的促炎标志物）的表达。
与对照组相比，中风患者发病后24小时内血清miR-146b表达显著升高。此外，血清miR-146b的上调与患者血浆hs-CRP、梗死体积及美国国立卫生研究院卒中量表（NIHSS）评分呈强正相关，与血清IL-6呈中度正相关。重要的是，血浆hs-CRP与血清miR-146b联合对AIS预测具有更好的敏感性/特异性（曲线下面积从0.782至0.863）。
我们的初步研究结果表明，急性缺血性中风患者血清miR-146b上调可能是评估AIS的潜在生物标志物。
