Group of Studies on Crustacean Biology, Ecology and Culture (NEBECC), University of the State of São Paulo (UNESP), Institute of Biosciences, Campus of Botucatu, Zoology Sector, 18618-970, Botucatu, State of São Paulo, Brazil.
Invertebrate Morphology Laboratory (LMI), University of the State of São Paulo (UNESP), School of Agricultural and Veterinarian Sciences, Department of Applied Biology, Campus of Jaboticabal, 14884-900, Jaboticabal, State of São Paulo, Brazil.
Arthropod Struct Dev. 2021 Jul;63:101060. doi: 10.1016/j.asd.2021.101060. Epub 2021 May 25.
We evaluated the mating behavior of Potimirim potimirim in the laboratory based on the assumption that this caridean might be hermaphroditic. We also performed histology and scanning electron microscopy analyses of the reproductive system of females, males, and differentiated individuals. The mating experiments produced three behavioral stages, namely, interaction, lateral positioning, and copulation (which did not necessarily occur in this sequence). The hypothesis of a random pure searching mating was corroborated by the lack of male courtship, postcopulatory guarding, and the high aggregation of individuals. Three macroscopic ovarian stages were recorded in adult females: rudimentary, developing and mature. Secondary vitellogenesis begins at the developing stage, producing mature yolk. The male reproductive system is formed by testes and the vasa deferentia (VD), that is divided into three regions: proximal, middle, and distal. The proximal VD has a typhlosole that produces a thin layer of type II secretion around the central spermatozoa mass immersed in a type I secretion. External and adherent type III secretion is produced from the MVD to DVD, and both compound the primordial spermatophore. Potimirim potimirim has a gonochoric reproductive system and mating behavior and its sexual system does not fit into any of the previously described protandric systems.
我们基于以下假设，在实验室中评估了 Potimirim potimirim 的交配行为，即这种虾可能是雌雄同体的。我们还对雌性、雄性和分化个体的生殖系统进行了组织学和扫描电子显微镜分析。交配实验产生了三个行为阶段，即互动、侧向定位和交配（不一定按此顺序发生）。缺乏雄性求偶、交配后守护和个体高度聚集证实了随机纯搜索交配的假设。成年雌虾记录了三个宏观卵巢阶段：原始、发育和成熟。次级卵黄发生始于发育阶段，产生成熟的卵黄。雄性生殖系统由睾丸和输精管（VD）组成，输精管分为近端、中端和远端三个区域。近端 VD 有一个螺旋线，在浸入 I 型分泌液中的中央精子团周围产生一层薄的 II 型分泌液。外部和附着的 III 型分泌液从 MVD 到 DVD 产生，两者都构成原始精荚。Potimirim potimirim 具有雌雄异体的生殖系统和交配行为，其性系统不符合以前描述的任何一种先雄型系统。
