Department of Physical Education and Sports Sciences, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
Department of Physical Education and Sports Sciences, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
Cytokine. 2021 Aug;144:155590. doi: 10.1016/j.cyto.2021.155590. Epub 2021 May 25.
Multiple sclerosis (MS) is the most common non-traumatic neurological cause of disability in young adults. Physical activity, particularly exercise training, is an evidence-based approach to managing symptoms, restoring function, and improving overall wellness in people with MS. As well, the use of probiotics can be effective in reducing the damage from inflammation in MS patients.
The study aimed to address changes in leptin, serotonin, and cortisol following eight weeks of aerobic exercise along with probiotic intake in a cuprizone-induced demyelination mouse model of MS.
Mice were exposed to cuprizone for 12 weeks. After 5 weeks, beam and performance tests were performed on them. The mice (n = 5 per group) were randomly divided into five groups: control (C), MS, MS with exercise (MS + Exe), MS with probiotic (MS + Prob), and MS with probiotic and exercise (MS + Prob + Exe). Exercise groups performed aerobic exercises 5 days a week, 10 min in the first week, 20 min in the second week, and 30 min daily in the third week until the eighth week. In the probiotic groups, the mice received probiotic by gavage. They were sacrificed after 3 months. Biochemical and molecular biology analyses were performed.
The results showed that leptin gene expression values in the MS + Prob + Exe, MS + Prob, and MS + Exe groups showed a decrease compared to the MS group, but the reduction was not significant (p > 0.05). Also, the leptin Elisa test in these intervention groups showed a significant decrease (P < 0.05). The serotonin gene expression values in the MS + Prob + Exe, MS + Prob, and MS + Exe groups were increased compared to the MS group, but the increase was not significant (p > 0.05). Furthermore, the serotonin Elisa test in these intervention groups showed a significant increase (P < 0.05). The cortisol Elisa test values in the MS + Exe and MS + Prob groups exhibited a decrease compared to the MS group, but the reduction was not significant (p > 0.05).
Overall, these results suggest that lifestyle interventions can be effective in improving pathological factors in patients with MS.
多发性硬化症（MS）是年轻人中最常见的非外伤性神经功能障碍原因。体育活动，特别是运动训练，是一种基于证据的方法，可以用于管理症状、恢复功能和改善 MS 患者的整体健康。此外，益生菌的使用可以有效地减轻 MS 患者炎症引起的损伤。
本研究旨在探讨在杯状朊病毒诱导的 MS 脱髓鞘小鼠模型中，进行八周有氧运动和摄入益生菌后，瘦素、血清素和皮质醇的变化。
将小鼠暴露于杯状朊病毒中 12 周。在第 5 周后，对它们进行了梁和性能测试。将小鼠（每组 5 只）随机分为五组：对照组（C）、MS 组、MS 运动组（MS+Exe）、MS 益生菌组（MS+Prob）和 MS 益生菌和运动组（MS+Prob+Exe）。运动组每周运动 5 天，第 1 周运动 10 分钟，第 2 周运动 20 分钟，第 3 周每天运动 30 分钟，直到第 8 周。在益生菌组中，通过灌胃给予益生菌。3 个月后处死小鼠。进行生化和分子生物学分析。
结果显示，MS+Prob+Exe、MS+Prob 和 MS+Exe 组的瘦素基因表达值与 MS 组相比有所下降，但差异无统计学意义（p>0.05）。此外，这些干预组的瘦素 Elisa 检测结果显示出显著下降（P<0.05）。MS+Prob+Exe、MS+Prob 和 MS+Exe 组的血清素基因表达值与 MS 组相比有所增加，但差异无统计学意义（p>0.05）。此外，这些干预组的血清素 Elisa 检测结果显示出显著增加（P<0.05）。MS+Exe 和 MS+Prob 组的皮质醇 Elisa 检测值与 MS 组相比有所下降，但差异无统计学意义（p>0.05）。
总体而言，这些结果表明生活方式干预可以有效改善 MS 患者的病理因素。
