鸡源携带 bla 基因的替加环素耐药肺炎克雷伯菌的出现。
Emergence of bla-bearing tigecycline-resistant Klebsiella aerogenes of chicken origin.
机构信息
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province, P.R. China; Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P.R. China.
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province, P.R. China.
出版信息
J Glob Antimicrob Resist. 2021 Sep;26:66-68. doi: 10.1016/j.jgar.2021.04.028. Epub 2021 May 26.
OBJECTIVES
The aim of this study was to characterise a tigecycline-resistant bla-bearing Klebsiella aerogenes strain (HNHF1) of chicken origin.
METHODS
Strain HNHF1 was characterised by phenotypic antimicrobial susceptibility testing, PCR, conjugation assays, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), whole-genome sequencing and bioinformatics analysis.
RESULTS
The bla gene was located on an IncHI2 plasmid (pHNHF1_NDM-9) carrying various antimicrobial resistance genes. Moreover, the genetic context ΔISAba125-bla-ble-trpF is similar to other bla-bearing genetic contexts. TA cloning experiments showed that tet(A) variants may play a partial role in high-level tigecycline resistance in HNHF1.
CONCLUSION
This is the first report of a tigecycline-resistant bla-bearing IncHI2 plasmid in a K. aerogenes ST4 isolate of animal origin, which poses a great threat to public health. Further comprehensive surveillance is needed.
目的
本研究旨在对一株源于鸡的携带 bla 基因的耐替加环素肺炎克雷伯菌（HNHF1）进行表型抗菌药物敏感性试验、PCR、接合试验、S1 核酸酶脉冲场凝胶电泳（S1-PFGE）、全基因组测序和生物信息学分析。
方法
对 HNHF1 菌株进行了特征描述。
结果
bla 基因位于一个 IncHI2 质粒（pHNHF1_NDM-9）上，该质粒携带多种抗菌药物耐药基因。此外，ISAba125-bla-ble-trpF 基因缺失序列与其他 bla 基因携带的遗传环境相似。TA 克隆实验表明，tet(A)变体可能在 HNHF1 高水平耐替加环素中起部分作用。
结论
这是首次报道源于动物的肺炎克雷伯菌 ST4 分离株中存在携带 bla 基因的 IncHI2 型质粒，对公共卫生构成了巨大威胁。需要进一步进行全面监测。
Zotero 插件