ABSL-III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, PR China.
Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, 510632, China.
Virology. 2021 Aug;560:76-85. doi: 10.1016/j.virol.2021.04.012. Epub 2021 May 7.
Chronically SHIV-infected cynomolgus monkeys were used to determine the effects of the antibody-mediated acute CD4 T cell depletion on viral load as well as on the immunological factors associated with disease progression. Compared with the control animals, CD4 T cell-depleted animals with SHIV infection showed (i) little alteration in plasma viral load over the period of 22 weeks after the depletion; (ii) increased CD4 T cell proliferation and turnover of macrophages at the early phase of the depletion, but subsequent decline to the basal levels; and (iii) little impact on the expression of the inflammatory cytokines and CC chemokines associated with disease progression. These findings indicate that the antibody-mediated acute CD4 T cell depletion had minimal impact on plasma viral load and disease progression in chronically SHIV-infected cynomolgus monkeys. Future investigations are necessary to identify the key factor(s) related to the immune activation and macrophage infection during the CD4 deletion in chronic viral infection.
慢性感染 SHIV 的食蟹猴被用于确定抗体介导的急性 CD4 T 细胞耗竭对病毒载量以及与疾病进展相关的免疫因素的影响。与对照动物相比，感染 SHIV 的 CD4 T 细胞耗竭动物表现出：（i）在耗竭后 22 周的时间内，血浆病毒载量几乎没有变化；（ii）在耗竭的早期阶段，CD4 T 细胞增殖和巨噬细胞更新增加，但随后下降到基础水平；（iii）对与疾病进展相关的炎症细胞因子和 CC 趋化因子的表达几乎没有影响。这些发现表明，抗体介导的急性 CD4 T 细胞耗竭对慢性感染 SHIV 的食蟹猴的血浆病毒载量和疾病进展几乎没有影响。需要进一步的研究来确定慢性病毒感染中 CD4 缺失期间与免疫激活和巨噬细胞感染相关的关键因素。
