Nutrition Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110054, India.
Respir Physiol Neurobiol. 2021 Sep;291:103695. doi: 10.1016/j.resp.2021.103695. Epub 2021 May 27.
We have compared the prophylactic efficacies of quercetin and salbutamol in preventing pulmonary surfactants oxidation under hypoxia. Male SD rats supplemented orally with quercetin (50 mg/Kg BW) and salbutamol (2 mg/Kg BW) were exposed to hypobaric hypoxia (7,620 m for 6 h). Hypoxia-mediated elevation in oxidative stress, inflammation, and extravasations of LDH & albumin content in BALF of rats were assessed. Western blotting and mRNA studies determined the differential expressions of Nrf-2, HO-1, and associated surfactant proteins (SP-A, SP-B, SP-C, & SP-D) in rat lungs. Later, the lung configuration under hypoxia was assessed histopathologically. Quercetin and salbutamol pretreatment considerably restored the expressions of Nrf-2, HO-1, and surfactant proteins to normal by attenuating the increase in oxidative stress, inflammation, and extravasations of plasma proteins in the animals under hypoxia. The histopathology has also evidenced the protective effect of quercetin in retaining normal lung architecture under hypoxia over salbutamol. The present study indicates the effectiveness of quercetin prophylaxis in preventing pulmonary surfactants oxidation under hypoxia over salbutamol.
我们比较了槲皮素和沙丁胺醇在预防缺氧下肺表面活性剂氧化中的预防效果。雄性 SD 大鼠经口补充槲皮素（50mg/Kg BW）和沙丁胺醇（2mg/Kg BW），然后暴露于低压缺氧环境（7620 米，6 小时）。评估缺氧介导的氧化应激、炎症和 BALF 中 LDH 和白蛋白含量的渗出增加。Western 印迹和 mRNA 研究确定了 Nrf-2、HO-1 和相关肺表面活性剂蛋白（SP-A、SP-B、SP-C 和 SP-D）在大鼠肺部的差异表达。随后，对缺氧下的肺结构进行组织病理学评估。槲皮素和沙丁胺醇预处理通过减轻动物缺氧下氧化应激、炎症和血浆蛋白渗出的增加，显著恢复了 Nrf-2、HO-1 和肺表面活性剂蛋白的表达，使之恢复正常。组织病理学还证明了槲皮素在保持缺氧下正常肺结构方面优于沙丁胺醇的保护作用。本研究表明，槲皮素预防在预防缺氧下肺表面活性剂氧化方面优于沙丁胺醇。
