Reproductive Science & Advanced Bioinformatics Application & Research Centre, Inonu University, Malatya, Turkey.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynaecology, School of Medicine, Inonu University, Malatya, Turkey.
BJOG. 2021 Dec;128(13):2101-2109. doi: 10.1111/1471-0528.16782. Epub 2021 Jun 20.
To evaluate the impact of vaginal microbiota on pregnancy outcomes in women undergoing assisted reproduction.
A prospective cohort study.
A university-based assisted reproductive technology (ART) centre.
223 women undergoing ART treatment.
Prior to embryo transfer, vaginal samples were collected from the posterior fornix. Vaginal microbiota identification was carried out using next-generation sequencing and categorised according to the V3-V4 hypervariable region in the 16S rRNA gene region.
ART clinical outcomes (implantation, clinical pregnancy rates and live birth rates).
The live birth rate in women with community state type (CST)-I (39%) was higher than that in women with CST-III (21.5%) but the difference was not statistically significant (P = 0.052). The relative abundance of Lactobacillus was lower in women who failed to become pregnant (NP group) (67.71%) than in women who became pregnant (PR group) (79.72%). However, this difference was not statistically significant (P = 0.06). In the NP group, the relative abundance of Streptococcus (7.81%) and Gardnerella (9.40%) was higher than that in the PR group (relative abundance of Streptococcus and Gardnerella was 2.28% and 5.56%, respectively). The abundance of Streptococcus was found to be statistically significantly different between the two study groups (P = 0.014). Linear discriminant analysis (LDA) further validated that Streptococcus had the highest contribution (LDA score >4.0) to the difference between these two groups.
Streptococcus has the highest contribution to the distinction between the PR and NP groups.
A relatively high abundance of Streptococcus in the vaginal microbiota may be associated with a lower ART success rate.
评估阴道微生物群对接受辅助生殖技术（ART）治疗的女性妊娠结局的影响。
前瞻性队列研究。
一家大学附属的 ART 中心。
223 名接受 ART 治疗的女性。
在胚胎移植前，从后阴道穹窿采集阴道样本。使用下一代测序技术对阴道微生物群进行鉴定，并根据 16S rRNA 基因区域的 V3-V4 高变区进行分类。
ART 临床结局（着床率、临床妊娠率和活产率）。
社区状态类型（CST）-I 组（39%）的活产率高于 CST-III 组（21.5%），但差异无统计学意义（P=0.052）。未妊娠（NP）组（67.71%）中乳杆菌的相对丰度低于妊娠（PR）组（79.72%），但差异无统计学意义（P=0.06）。然而，NP 组中链球菌（7.81%）和加德纳菌（9.40%）的相对丰度高于 PR 组（链球菌和加德纳菌的相对丰度分别为 2.28%和 5.56%）。两组间链球菌的丰度差异有统计学意义（P=0.014）。线性判别分析（LDA）进一步验证了链球菌对两组间差异的贡献最大（LDA 评分>4.0）。
链球菌对 PR 组和 NP 组的区分贡献最大。
