School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
Institute of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.
Food Res Int. 2021 Jun;144:110323. doi: 10.1016/j.foodres.2021.110323. Epub 2021 Mar 22.
Health Tonic oral liquid (HT) is a popular functional food in China and is used to enhance host immune response. However, its mechanisms of action are still poorly understood. In this work, we combined ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS) serum metabolomics with 16S rDNA sequencing to evaluate the effects of HT on metabolomics profiling and microbial community signatures. Short-chain fatty acids (SCFAs) contents in fecal were quantified through gas chromatography-mass spectrometry (GC-MS). Results indicated that HT use leads to a significant increase in IgG, IgM and IgA. Thirty-four metabolites were identified and quantified using metabolomics, most were aromatic amino acids and metabolites involved in glucose metabolism. HT intervention significantly increased the abundance of Alloprevotella, which may contribute to intestinal barrier integrity and inflammatory response inhabitation. Most SCFAs were highly expressed following HT intake. In summary, HT use maintains glucose and lipid metabolism balance, promotes high expressions of beneficial bacteria, and exerts promising immunomodulatory effects.
养生口服液（HT）是中国一种颇受欢迎的功能食品，用于增强机体免疫应答。然而，其作用机制仍知之甚少。本研究采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q/TOF MS）血清代谢组学结合 16S rDNA 测序，评估 HT 对代谢组学特征和微生物群落特征的影响。采用气相色谱-质谱（GC-MS）法对粪便中短链脂肪酸（SCFAs）含量进行定量。结果表明，HT 使用导致 IgG、IgM 和 IgA 显著增加。采用代谢组学鉴定和定量了 34 种代谢物，其中大多数为芳香族氨基酸和参与葡萄糖代谢的代谢物。HT 干预显著增加了 Alloprevotella 的丰度，这可能有助于肠道屏障完整性和炎症反应抑制。大多数 SCFAs 在 HT 摄入后呈高表达。综上所述，HT 使用可维持糖脂代谢平衡，促进有益菌的高表达，并发挥有前景的免疫调节作用。
