Department of Clinical Laboratory, Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital, Taiyuan, China.
Department of Neonatology Department, Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital, Taiyuan, China.
Front Cell Infect Microbiol. 2021 May 14;11:642455. doi: 10.3389/fcimb.2021.642455. eCollection 2021.
Group B streptococcus (GBS) is a leading cause of serious infections in infants. The extensive use of tetracycline has led to the selection of specific resistant and infectious GBS clones. The sequence type (ST) 10 GBS strain, causing invasive infections in infants, is becoming prevalent in China. We aimed to understand the clinical and microbiological characteristics of this GBS strain.
We conducted a retrospective study on infants with invasive GBS disease from the largest women's and children's medical center in Shanxi and collected data between January 2017 and October 2020. GBS isolates were analyzed by capsule serotyping, genotyping, antibiotic resistance, and surface protein genes.
All ST10 isolates belonged to serotype Ib; type Ib/ST10 strains were responsible for 66.7% (14/21, < 0.05) of infant invasive GBS infections during the period and all resulted in late-onset (LOD) and late LOD disease (14/14). Infants with type Ib/ST10 GBS disease had significantly higher rates of meningitis (9/14, 64.3%, p < 0.05) and clinical complications (5/14, 35.7%, p < 0.05). The Ib/ST10 GBS isolates had limited genetic diversity, clustered in the CC10/bca/PI-1 + PI-2a genetic lineage, showed resistance to erythromycin, lincomycin, and fluoroquinolones and sensitivity to tetracycline, and possessed genes , , and amino acid changes in and .
The probable clonal expansion can result in severe infections in infants and ongoing emergence of multi-drug resistant isolates. Continued monitoring for type Ib/ST10 GBS infections is warranted.
B 群链球菌（GBS）是导致婴儿严重感染的主要原因。四环素的广泛使用导致了特定的耐药和传染性 GBS 克隆的选择。引起婴儿侵袭性感染的 10 型 STGBS 菌株在中国越来越普遍。我们旨在了解该 GBS 菌株的临床和微生物学特征。
我们对山西最大的妇女儿童医院的侵袭性 GBS 疾病婴儿进行了回顾性研究，并在 2017 年 1 月至 2020 年 10 月期间收集数据。通过荚膜血清型、基因分型、抗生素耐药性和表面蛋白基因分析 GBS 分离株。
所有 ST10 分离株均属于 Ib 型；Ib/ST10 菌株在该时期导致 66.7%（14/21，<0.05）的婴儿侵袭性 GBS 感染，均为晚发型（LOD）和晚 LOD 疾病（14/14）。患有 Ib/ST10 GBS 疾病的婴儿脑膜炎（9/14，64.3%，p<0.05）和临床并发症（5/14，35.7%，p<0.05）的发生率明显更高。Ib/ST10 GBS 分离株遗传多样性有限，聚集在 CC10/bca/PI-1+PI-2a 遗传谱系中，对红霉素、林可霉素和氟喹诺酮类药物耐药，对四环素敏感，并且具有 和 基因， 和 氨基酸变化。
可能的克隆扩张可导致婴儿严重感染和持续出现多药耐药分离株。有必要持续监测 Ib/ST10 GBS 感染。
