Liu Zeliang, Jiang Xueqi, Li Jie, Ji Wenjing, Zhou Haijian, Gong Xinyi, Miao Beibei, Meng Shuang, Duan Like, Shi Qiyuan, Han Xiao, Gao Pengfang, Chang Chienyi, Dong Aiying, Li Juan
Affiliated Hospital of North China University of Science and Technology, Tangshan, Hebei, China.
State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Front Microbiol. 2023 Mar 1;14:1138039. doi: 10.3389/fmicb.2023.1138039. eCollection 2023.
(Group B , GBS) is a major cause of neonatal infections with high morbidity and mortality, and clindamycin is the main antibiotic used to treat GBS infections in patients allergic to penicillin. We aimed to analyse the antibiotic sensitivity, sequence types, serotypes, virulence factors, and antibiotic resistance mechanisms of clinically isolated clindamycin-resistant and provide basic data for the treatment, prevention, and control of clinical infection of . A total of 110 strains of clindamycin-resistant were collected from two tertiary hospitals in Hebei, China. We performed antibiotic sensitivity tests for 11 antibiotics on these strains and whole-genome sequencing analysis. All the strains were susceptible to penicillin, ampicillin, linezolid, vancomycin, tigecycline, and quinupristin-dalfopristin. Resistance to erythromycin, levofloxacin, tetracycline, and chloramphenicol were also observed. Genome sequence analysis revealed that all strains belonged to 12 sequence types (STs) related to six cloning complexes (CCs), namely CC10, CC19, CC23, CC651, CC1, and CC17. Five serotypes were identified, including IA, IB, II, III, and V. The most prominent resistance genes were (100%) and (81.8%). Furthermore, , , and the gene cluster related to the pili were 100% present in all strains, followed by (95.5%) and (67.2%). This study found that clindamycin-resistant showed polymorphisms in molecular types and serotypes. Furthermore, multiple virulence factor genes have been identified in their genomes.
B组链球菌（GBS）是新生儿感染的主要病因，发病率和死亡率很高，对于对青霉素过敏的患者，克林霉素是治疗GBS感染的主要抗生素。我们旨在分析临床分离的耐克林霉素GBS的抗生素敏感性、序列类型、血清型、毒力因子和抗生素耐药机制，为GBS临床感染的治疗、预防和控制提供基础数据。从中国河北的两家三级医院收集了总共110株耐克林霉素GBS。我们对这些菌株进行了11种抗生素的药敏试验和全基因组测序分析。所有菌株对青霉素、氨苄西林、利奈唑胺、万古霉素、替加环素和奎奴普丁-达福普汀敏感。也观察到对红霉素、左氧氟沙星、四环素和氯霉素的耐药性。基因组序列分析表明，所有菌株属于与6个克隆复合体（CCs）相关的12种序列类型（STs），即CC10、CC19、CC23、CC651、CC1和CC17。鉴定出5种血清型，包括IA、IB、II、III和V。最突出的耐药基因是erm（B）（100%）和msr（A）（81.8%）。此外，sagA、bca、scpB和与菌毛相关的基因簇在所有菌株中均100%存在，其次是hasA（95.5%）和covR（67.2%）。本研究发现，耐克林霉素GBS在分子类型和血清型上表现出多态性。此外，在其基因组中鉴定出多个毒力因子基因。
