EPSRC Centre for Doctoral Training, Centre for Sustainable Chemical Technologies, University of Bath, Bath, UK.
Department of Chemical Engineering, University of Bath, Bath, UK.
J Biomed Mater Res A. 2021 Dec;109(12):2471-2482. doi: 10.1002/jbm.a.37241. Epub 2021 May 31.
Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.
支架材料适合规模化和随后的组织工程产品的商业化，应该具有成本效益和可及性。对于某些贴壁细胞的体外培养，通常采用合成制造技术来生产微或纳米图案化的基底，通过接触引导的机制来影响细胞附着、形态和排列。在这里，我们提出了一种天然支架，以脱细胞化的观赏草的形式存在，它保留了其自然的条纹地形，并支持鼠 C2C12 成肌细胞的附着、增殖、排列和分化，而不需要额外的功能化。这为组织工程应用提供了一种廉价、可持续的支架材料和结构，能够影响细胞排列，这是培养骨骼肌和其他各向异性组织的理想特性。
