College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, 210023, Jiangsu, China.
Nanjing Institute for Food and Drug Control, Nanjing, 211198, Jiangsu, China.
Anal Bioanal Chem. 2021 Jul;413(16):4277-4287. doi: 10.1007/s00216-021-03379-4. Epub 2021 May 31.
A novel smartphone-based electrochemical cell sensor was developed to evaluate the toxicity of heavy metal ions, such as cadmium (Cd), lead (Pb), and mercury (Hg) ions on Hep G2 cells. The cell sensor was fabricated with reduced graphene oxide (RGO)/molybdenum sulfide (MoS) composites to greatly improve the biological adaptability and amplify the electrochemical signals. Differential pulse voltammetry (DPV) was employed to measure the electrical signals induced by the toxicity of heavy metal ions. The results showed that Cd, Hg, and Pb significantly reduced the viability of Hep G2 cells in a dose-dependent manner. The IC values obtained by this method were 49.83, 36.94, and 733.90 μM, respectively. A synergistic effect was observed between Cd and Pb and between Hg and Pb, and an antagonistic effect was observed between Cd and Hg, and an antagonistic effect at low doses and an additive effect at high doses were found in the ternary mixtures of Cd, Hg, and Pb. These electrochemical results were confirmed via MTT assay, SEM and TEM observation, and flow cytometry. Therefore, this new electrochemical cell sensor provided a more convenient, sensitive, and flexible toxicity assessment strategy than traditional cytotoxicity assessment methods.
一种新型基于智能手机的电化学池传感器被开发出来，用于评估重金属离子（如镉（Cd）、铅（Pb）和汞（Hg）离子）对 Hep G2 细胞的毒性。该细胞传感器采用还原氧化石墨烯（RGO）/硫化钼（MoS）复合材料制造，以大大提高生物适应性并放大电化学信号。差分脉冲伏安法（DPV）用于测量重金属离子毒性引起的电信号。结果表明，Cd、Hg 和 Pb 以剂量依赖性方式显著降低 Hep G2 细胞的活力。该方法获得的 IC 值分别为 49.83、36.94 和 733.90 μM。Cd 和 Pb 之间以及 Hg 和 Pb 之间观察到协同作用，而 Cd 和 Hg 之间观察到拮抗作用，在 Cd、Hg 和 Pb 的三元混合物中，在低剂量下观察到拮抗作用，在高剂量下观察到相加作用。这些电化学结果通过 MTT 测定、SEM 和 TEM 观察以及流式细胞术得到证实。因此，与传统的细胞毒性评估方法相比，这种新型电化学池传感器提供了更方便、更灵敏和更灵活的毒性评估策略。
