Stevens W C, Huang D H, Wells R D, Krishna N R
Department of Biochemistry, School of Medicine, University of Alabama, Birmingham 35294.
Nucleic Acids Res. 1988 Jul 25;16(14B):7133-43. doi: 10.1093/nar/16.14.7133.
The deoxyoligonucleotide d(TGCA)3 is a candidate for exhibiting unusual conformations. Its 1H NMR spectrum under low salt conditions has been obtained at 400 MHz and assigned using two-dimensional NMR techniques. The sugar puckers and glycosidic torsions have been determined by inspecting the relative intensities of the intranucleotide NOEs and COSY crosspeaks. At low electrolyte concentration (100 mM NaCl) the molecule exists as a right-handed duplex with twelve Watson-Crick base-pairs and deoxyribose moieties assuming the O1'-endo to C1'-exo pucker.
脱氧寡核苷酸d(TGCA)3是呈现异常构象的候选分子。已在400 MHz下获得其在低盐条件下的1H NMR谱,并使用二维NMR技术进行了归属。通过检查核苷酸内NOE和COSY交叉峰的相对强度确定了糖的折叠和糖苷扭转。在低电解质浓度(100 mM NaCl)下,该分子以具有十二个沃森-克里克碱基对的右手双链体形式存在,脱氧核糖部分呈现O1'-内向至C1'-外向折叠。