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在500兆赫频率下对5S核糖核酸的一种抗核糖核酸酶片段的低场质子光谱进行核Overhauser实验。

Nuclear Overhauser experiments at 500 MHz on the downfield proton spectrum of a ribonuclease-resistant fragment of 5S ribonucleic acid.

作者信息

Kime M J, Moore P B

出版信息

Biochemistry. 1983 May 24;22(11):2615-22. doi: 10.1021/bi00280a004.

DOI:10.1021/bi00280a004
PMID:6347249
Abstract

The downfield (9-15 ppm) proton NMR spectrum of a RNase A resistant fragment of E. coli 5S RNA has been studied by nuclear Overhauser methods. The fragment comprises bases 1-11 and 69-120 of the parent molecule [Douthwaite, S., Garrett, R.A., Wagner, R., & Feunteun, J. (1979) Nucleic Acids Res. 6, 2453-2470]. The nuclear Overhauser data identify two double helical structures in the fragment whose sequences are (GC)3(AU)(GC)3 and (GC)2(AU)(GU). These structures correspond exactly to the central portions of the terminal stem and procaryotic loop helices which should exist in the fragment sequences according to the Fox-Woese model [Fox, G.E., & Woese, C. R. (1975) Nature (London) 256, 505-506] of 5S RNA secondary structure. The significance of these and other nuclear Overhauser effects detected for the structure of 5S RNA and its fragment is discussed.

摘要

通过核Overhauser方法研究了大肠杆菌5S RNA的核糖核酸酶A抗性片段的低场（9 - 15 ppm）质子核磁共振谱。该片段包含亲本分子的第1 - 11位和69 - 120位碱基[杜思韦特，S.，加勒特，R.A.，瓦格纳，R.，& 丰特恩，J.（1979年）《核酸研究》6，2453 - 2470]。核Overhauser数据确定了该片段中的两个双螺旋结构，其序列分别为(GC)3(AU)(GC)3和(GC)2(AU)(GU)。根据5S RNA二级结构的福克斯 - 沃斯模型[福克斯，G.E.，& 沃斯，C.R.（1975年）《自然》（伦敦）256，505 - 506]，这些结构恰好对应于片段序列中应该存在的末端茎和原核环螺旋的中心部分。讨论了这些以及为5S RNA及其片段结构检测到的其他核Overhauser效应的意义。

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