CNRS ERL7001 LNOx "Leukemic Niche & Redox Metabolism", Tours, France.
EA7501 GICC, Faculty of Medicine, Tours University, Tours, France.
Methods Mol Biol. 2021;2308:59-70. doi: 10.1007/978-1-0716-1425-9_5.
Bone marrow mesenchymal stromal cells (MSCs) play an essential role in the regulation of normal and leukemic hematopoiesis. Their multipotent potential of differentiation also makes them an interesting therapeutic tool. Among factors involved in the regulation of MSCs, energy metabolism plays a key role in their proliferation and differentiation. Seahorse Bioscience introduced extracellular flux technology to the life sciences market in 2006. This methodology allows, in living cells and in real time, the concomitant determination of basal oxygen consumption, glycolysis rates, ATP production, and respiratory capacity in a single experiment. Here we describe the protocol used to study concomitantly the respiratory and glycolytic metabolism of primary MSCs from the determination of oxygen consumption (OCR) and extracellular acidification (ECAR) rates.
骨髓间充质基质细胞 (MSCs) 在调节正常和白血病造血中起着至关重要的作用。它们的多能分化潜力也使它们成为一种有趣的治疗工具。在调节 MSCs 的因素中，能量代谢在其增殖和分化中起着关键作用。 Seahorse Bioscience 于 2006 年将细胞外通量技术引入生命科学市场。该方法允许在单个实验中实时同时测定活细胞中的基础耗氧量、糖酵解率、ATP 产生和呼吸能力。在这里，我们描述了用于同时研究原发性 MSCs 的呼吸和糖酵解代谢的方案，从氧消耗 (OCR) 和细胞外酸化 (ECAR) 率的测定开始。
