Tripathi Yogesh, Shukla Mukul, Bhatt Amba D
CAD Laboratory, Department of Mechanical Engineering, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, Allahabad, UP, India.
Proc Inst Mech Eng H. 2021 Sep;235(9):1025-1034. doi: 10.1177/09544119211022988. Epub 2021 May 31.
Porous scaffolds assisted bone tissue engineering is a viable alternative for reconstruction of large segmental bone defects caused by bone pathologies or trauma. In the current study, we intend to develop trabecular bone scaffolds using gyroid architecture. An interactive modeling framework is developed for the design of three-dimensional gyroid scaffolds using advanced generative tools including K3DSurf, MeshLab, and Netfabb. The suggested modeling approach resulted in uniform and interconnected pores. Subsequently, fused deposition modeling 3D-printing is employed to fabricate the scaffolds using poly lactic acid material. The pores interconnectivity, porosity, and surface finish of the fabricated scaffolds are characterized using micro-computer tomography and scanning electron microscopy. Additionally, to assess the performance of scaffolds as a bone substitute, compression, and in-vitro biocompatibility tests on sterilized scaffolds are conducted. Compression tests reveal mechanical strength in the range of native bone while human adipose-derived mesenchymal stem cells show high proliferation after 72 h of incubation. Based on these results, the fabricated gyroid scaffolds can be said to possess favorable properties for trabecular bone scaffold.
多孔支架辅助骨组织工程是一种可行的替代方法，用于重建由骨病变或创伤引起的大段骨缺损。在当前研究中，我们打算使用类螺旋结构开发小梁骨支架。利用包括K3DSurf、MeshLab和Netfabb在内的先进生成工具，开发了一个交互式建模框架，用于设计三维类螺旋支架。所建议的建模方法产生了均匀且相互连通的孔隙。随后，采用熔融沉积建模3D打印技术，使用聚乳酸材料制造支架。使用微型计算机断层扫描和扫描电子显微镜对制造的支架的孔隙连通性、孔隙率和表面光洁度进行表征。此外，为了评估支架作为骨替代物的性能，对灭菌后的支架进行了压缩和体外生物相容性测试。压缩测试显示其机械强度在天然骨的范围内，而人脂肪来源的间充质干细胞在培养72小时后显示出高增殖率。基于这些结果，可以说制造的类螺旋支架具有作为小梁骨支架的良好特性。
