Chang H C, Jones O W
Department of Medicine, University of California, San Diego, La Jolla 92093.
Prenat Diagn. 1988 Jun;8(5):367-78. doi: 10.1002/pd.1970080507.
In vitro characteristics of human fetal cells have been investigated after chorionic villus sampling at the first trimester and amniocentesis at the second trimester of pregnancy. Light microscopy revealed heterogeneous morphology of cell types in both the chorionic villus culture and the amniotic fluid cultures. Based on the experiments performed, chorionic villus cells are more sensitive to pronase, trypsin, and versene during subculture and have a higher DNA content per single cell and release more [125I]-Beta-human chorionic gonadotropin into culture medium than those found in amniotic fluid cells. The practical applications of this study are discussed.
在妊娠早期进行绒毛取样和妊娠中期进行羊膜穿刺术后，对人胎儿细胞的体外特性进行了研究。光学显微镜显示，绒毛膜绒毛培养物和羊水培养物中的细胞类型形态各异。根据所进行的实验，绒毛膜绒毛细胞在传代培养过程中对链霉蛋白酶、胰蛋白酶和乙二胺四乙酸更为敏感，单个细胞的DNA含量更高，并且比羊水细胞向培养基中释放更多的[125I]-β-人绒毛膜促性腺激素。讨论了本研究的实际应用。
