Wright D J, Brindley B A, Koppitch F C, Drugan A, Johnson M P, Evans M I
Department of Obstetrics/Gynecology, Hutzel Hospital/Wayne State University, Detroit, Michigan.
Obstet Gynecol. 1989 Nov;74(5):739-44.
Karyotypes of chorionic villi have been said to be less accurate than karyotypes of amniocytes. Karyotypic differences between placental and fetal tissue and maternal-cell contamination could potentially complicate clinical management. We compared cytogenetic results obtained by chorionic villus sampling and amniotic fluid cells in our center during a 2-year period (1986-1987). Chorionic villus sampling material was processed for direct analysis and backed up when indicated (now routinely) with tissue cultures. The incidence of inconclusive results requiring additional studies was 1.2% for chorionic villus sampling and 0.75% for amniotic fluid cells. Mosaicism was the most common problem for both chorionic villus sampling and amniotic fluid cells. Failure of growth was more frequent in amniocentesis material (0.35 versus 0.09%), but polyploidy and atypical aneuploidies were greater with chorionic villus sampling. The accuracy of cytogenetic evaluation by chorionic villus sampling and amniotic fluid cells and the need for additional invasive procedures appear to be equal in our laboratory.
有人认为绒毛膜绒毛的核型分析不如羊水细胞的核型分析准确。胎盘组织与胎儿组织之间的核型差异以及母细胞污染可能会使临床处理复杂化。我们比较了本中心在1986年至1987年这两年期间通过绒毛取样和羊水细胞获得的细胞遗传学结果。绒毛取样材料经处理后直接进行分析，并在必要时（现在常规进行）通过组织培养备份。绒毛取样需要进一步研究的不确定结果发生率为1.2%，羊水细胞为0.75%。嵌合体是绒毛取样和羊水细胞最常见的问题。羊水穿刺材料生长失败更为常见（0.35%对0.09%），但绒毛取样的多倍体和非典型非整倍体更多。在我们实验室，绒毛取样和羊水细胞的细胞遗传学评估准确性以及额外侵入性操作的必要性似乎相当。
