Rotating Disc B.V., Spoorlaan 31, 9753 HV Haren, The Netherlands.
Eurofins Expertise Centre for Complex Carbohydrates and Chemistry, P.O. Box 766, 8440 AT Heerenveen, The Netherlands.
J AOAC Int. 2021 Jun 12;104(3):732-756. doi: 10.1093/jaoacint/qsaa092.
In this international Multi-Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose, and maltose) in milk and milk products with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established.
Thirteen different laboratories participated (located in New Zealand, the United States, and Europe). The study was divided into two parts. First, the learning/familiarization part in which the participants got experienced with the method. After successfully completing this part, the participants were allowed to continue with the second part of this trial: the analyses of the seven blind MLT duplicate samples.
The selected seven MLT samples comprise five MUVA reference dairy samples, one NIST-certified infant formula and one industrial sample. In the Tables S-1 to S-6 the results of this MLT study have been summarized per sugar. There is a very good agreement between the MUVA and NIST-certified sugar contents in the MLT samples and in the MLT established sugar contents in those MUVA and NIST samples.
This demonstrates that the accuracy of the newly developed HPAEC-PAD method is very good.
Certificates of analysis were available for both the MUVA samples and the NIST sample. There is a very good agreement between the by MUVA- and NIS-certified sugar contents in the MLT samples and the corresponding in the MLT established sugar contents. This demonstrates that the accuracy of the HPAEC-PAD method is very good. In the IDF/ISO Analytical Week in June 2019 in Prague the report on the MLT study were discussed and adopted.
在这项国际多实验室试验（MLT）中，使用高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）对牛奶和奶制品中 6 种不同单糖和二糖（半乳糖、葡萄糖、果糖、蔗糖、乳糖和麦芽糖）进行定量的 ISO/CD 22184 IDF/WD 244 分析方法的精密度数据和准确性得到了确立。
共有 13 个不同的实验室参与（分别位于新西兰、美国和欧洲）。该研究分为两部分。首先，参与者在学习/熟悉部分中获得了方法的经验。成功完成这部分后，参与者被允许继续进行试验的第二部分：对 7 个盲 MLT 重复样品的分析。
所选的 7 个 MLT 样品包括 5 个 MUVA 参考乳制品样品、1 个 NIST 认证婴儿配方奶粉和 1 个工业样品。在表 S-1 至 S-6 中，按糖总结了这项 MLT 研究的结果。在 MLT 样品中，MUVA 和 NIST 认证的糖含量以及在 MUVA 和 NIST 这些样品中的 MLT 建立的糖含量之间有很好的一致性。
这表明新开发的 HPAEC-PAD 方法的准确性非常好。
MUVA 样品和 NIST 样品都有分析证书。在 MLT 样品中，MUVA 和 NIST 认证的糖含量以及在 MLT 建立的糖含量之间有很好的一致性。这表明 HPAEC-PAD 方法的准确性非常好。在 2019 年 6 月布拉格的 IDF/ISO 分析周上，对 MLT 研究报告进行了讨论和通过。
