Molecular Biology & Microbial Food Safety, Swammerdam Institute for Life Sciences (SILS), Faculty of Science (FNWI), University of Amsterdam, Amsterdam, The Netherlands.
Academisch Medisch Centrum Universiteit van, Amsterdam, The Netherlands.
Methods Mol Biol. 2021;2276:397-407. doi: 10.1007/978-1-0716-1266-8_29.
Caenorhabditis elegans is a highly versatile model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.
秀丽隐杆线虫是一种用途广泛的模式生物系统，被广泛用于功能、遗传、细胞计量和综合研究。由于其结构简单，肌肉细胞数量多，秀丽隐杆线虫常被用于研究疾病或药物毒性引起的线粒体缺陷。在这里，我们描述了一种在体内可视化和定量线粒体形态的强大而有效的方法。与传统（手动）方法相比，该方法具有许多实际和技术优势，并提供了线粒体形态的全面分析。
