秀丽隐杆线虫中线粒体形态的体内可视化与定量分析。

In vivo visualization and quantification of mitochondrial morphology in C. elegans.

作者信息

Smith Reuben L, De Vos Winnok H, de Boer Richard, Manders Erik M M, van der Spek Hans

机构信息

Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences (SILS), Faculty of Science (FNWI), University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

出版信息

Methods Mol Biol. 2015;1265:367-77. doi: 10.1007/978-1-4939-2288-8_26.

DOI:10.1007/978-1-4939-2288-8_26

PMID:25634288

Abstract

Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.

摘要

秀丽隐杆线虫是一种具有高度可塑性的模型系统，广泛应用于功能、遗传、细胞计量和综合研究。由于其结构简单且肌肉细胞数量众多，秀丽隐杆线虫经常被用于研究由疾病或药物毒性引起的线粒体缺陷。在此，我们描述了一种在体内可视化和量化线粒体形态的强大而有效的方法。该方法相对于传统（手动）方法具有许多实际和技术优势，并能对线粒体形态进行全面分析。