Institute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 200092, China.
Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Shanghai, Tongji Univeirsity School of Medicine, Shanghai, China, China.
J Biomed Mater Res A. 2021 Oct;109(10):1849-1857. doi: 10.1002/jbm.a.37177. Epub 2021 Jun 1.
Extracellular vesicles from adipose derived stem cells (ADSCs-EVs) have shown immunomodulation and anti-photoaging effects; however, the skin barrier prevents their absorption via skin. Meanwhile, microneedle (MN) is a widely used and minimally invasive tool for dermal delivery of drugs, it also has neocollagenesis effect by creating tiny injuries and initiating wound healing process. To investigate the effect of MN combined with ADSCs-EVs on skin aging, photoaging in SKH-1 mice was induced by chronic exposure to ultraviolet radiation. Then the mice were treated following a split-dorsal scheme, in which one side had MN alone or MN + EVs treatment and the other side was left untreated. For the side treated with MN alone or MN + EVs, the epidermal thickness was decreased and the skin barrier function was enhanced compared with the untreated side. However, MN + EVs group showed the least wrinkles, the highest collagen density and the most organized collagen fibers among the three groups. The level of CD11b + cell infiltration was lower in MN + EVs group than that in the MN group at 3 day after the treatment. These results indicated that MN treatment alone could improve epidermal structure and function of photoaging skin, and a combination with ADSCs-EVs would accelerate the restoration of inflammation caused by MN and improve the content of collagen. In all, this study indicated that a combination of MN and topical applied ADSCs-EVs was a feasible and safe strategy to ameliorate photoaging, providing a new avenue for safe administration of EVs.
脂肪来源干细胞（ADSCs-EVs）的细胞外囊泡具有免疫调节和抗光老化作用；然而，皮肤屏障会阻止它们通过皮肤吸收。同时，微针（MN）是一种广泛使用的微创工具，可用于将药物递送至皮肤，它通过制造微小的损伤并启动伤口愈合过程，也具有新胶原生成的作用。为了研究 MN 联合 ADSCs-EVs 对皮肤老化的影响，通过慢性暴露于紫外线辐射诱导 SKH-1 小鼠光老化。然后，采用背部分割方案对小鼠进行治疗，一侧单独使用 MN 或 MN+EVs 治疗，另一侧不治疗。与未治疗侧相比，单独使用 MN 或 MN+EVs 治疗的侧表皮厚度减小，皮肤屏障功能增强。然而，MN+EVs 组的皱纹最少，胶原密度最高，胶原纤维最规整。与 MN 组相比，MN+EVs 组在治疗后 3 天 CD11b+细胞浸润水平较低。这些结果表明，MN 单独治疗可改善光老化皮肤的表皮结构和功能，与 ADSCs-EVs 联合使用可加速 MN 引起的炎症恢复并提高胶原含量。总之，这项研究表明，MN 和局部应用的 ADSCs-EVs 的联合使用是改善光老化的一种可行且安全的策略，为 EVs 的安全给药提供了新途径。
