原花青素 B2 通过 AMP 激活的蛋白激酶信号通路诱导猪骨骼肌慢肌纤维基因表达。
Procyanidin B2 induces porcine skeletal slow-twitch myofiber gene expression by AMP-activated protein kinase signaling pathway.
机构信息
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
College of Food Science, Sichuan Agricultural University, Yaan, Sichuan, P. R. China.
出版信息
Anim Biotechnol. 2022 Apr;33(2):346-355. doi: 10.1080/10495398.2021.1927065. Epub 2021 Jun 1.
In this study, our aim is to investigate the effect of dimer procyanidin B2 [epicatechin-(4β-8)-epicatechin] (PB2) on porcine skeletal myofiber gene expression . Our data showed PB2 promoted the protein expression of slow myosin heavy chain (MyHC) in porcine myotubes, concomitant with the increases in mRNA levels of , and . We also found PB2 activated AMPK signaling in porcine myotubes. NRF1 and CaMKKβ that are two important upstream factors of AMPK, and Sirt1 and PGC-1α that are two major downstream factors of AMPK, were also up-regulated by PB2. The mechanism study showed the effect of PB2 on slow-twitch myofiber gene expression was abolished by AMPK inhibitor compound C or by AMPKα1 siRNA. Together, we found PB2 induced porcine skeletal slow-twitch myofiber gene expression by AMPK signaling pathway.
在这项研究中，我们的目的是研究二聚原花青素 B2 [表儿茶素-(4β-8)-表儿茶素]（PB2）对猪骨骼肌肌纤维基因表达的影响。我们的数据表明，PB2 促进了猪肌管中慢肌肌球蛋白重链（MyHC）的蛋白表达，同时 、 和 的 mRNA 水平也增加。我们还发现 PB2 激活了猪肌管中的 AMPK 信号通路。NRF1 和 CaMKKβ 是 AMPK 的两个重要上游因子，Sirt1 和 PGC-1α 是 AMPK 的两个主要下游因子，它们也被 PB2 上调。机制研究表明，AMPK 抑制剂化合物 C 或 AMPKα1 siRNA 可消除 PB2 对慢收缩肌纤维基因表达的影响。总之，我们发现 PB2 通过 AMPK 信号通路诱导猪骨骼肌慢收缩肌纤维基因表达。
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