白藜芦醇通过 AMPK 信号通路和 miR-22-3p 调节猪肌管肌纤维类型基因表达。
Resveratrol regulates muscle fiber type gene expression through AMPK signaling pathway and miR-22-3p in porcine myotubes.
机构信息
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China.
College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, China.
出版信息
Anim Biotechnol. 2022 Jun;33(3):579-585. doi: 10.1080/10495398.2022.2046599. Epub 2022 Mar 10.
This study aimed to investigate the effect and underlying mechanisms of resveratrol on porcine muscle fiber type gene expression in porcine myotubes. Here, results showed that resveratrol treatment significantly promoted slow myosin heavy chain (MyHC) and inhibited fast MyHC in porcine myotubes. The phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and the downstream factors of AMPK signaling, such as Sirtuin1 (Sirt1) and peroxlsome proliferator-activated receptor-γ coactlvator-1α (PGC-1α), were also increased by resveratrol, suggesting that resveratrol could activate the AMPK signaling pathway. Interestingly, resveratrol inhibited the expression of miR-22-3p in porcine myotubes. Furthermore, AMPK inhibitor compound C and miR-22-3p mimic effectively eliminated the effects of resveratrol on slow MyHC and fast MyHC expressions in porcine myotubes. Taken together, our findings indicate that resveratrol regulates muscle fiber type gene expression through the AMPK signaling pathway and miR-22-3p in porcine myotubes.
本研究旨在探讨白藜芦醇对猪肌管中肌纤维型基因表达的影响及其潜在机制。结果表明，白藜芦醇处理可显著促进猪肌管中慢肌肌球蛋白重链（MyHC）的表达，抑制快肌 MyHC 的表达。白藜芦醇还可增加 AMP 激活的蛋白激酶（AMPK）及其下游信号因子如 Sirtuin1（Sirt1）和过氧化物酶体增殖物激活受体-γ 共激活因子-1α（PGC-1α）的磷酸化，提示白藜芦醇可激活 AMPK 信号通路。有趣的是，白藜芦醇可抑制猪肌管中 miR-22-3p 的表达。此外，AMPK 抑制剂化合物 C 和 miR-22-3p 模拟物可有效消除白藜芦醇对猪肌管中慢肌 MyHC 和快肌 MyHC 表达的影响。综上所述，本研究结果表明，白藜芦醇通过 AMPK 信号通路和猪肌管中的 miR-22-3p 调节肌纤维型基因表达。
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