Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146, USA.
Chemistry. 2021 Aug 5;27(44):11257-11267. doi: 10.1002/chem.202101628. Epub 2021 Jun 24.
The borondipyrromethene (BODIPY) chromophore is a versatile platform for the construction of photoresponsive dyes with unique properties. Specifically, its covalent connection to a photocleavable group can be exploited to engineer compounds with photoswitchable fluorescence. The resulting photoactivatable fluorophores can increase their emission intensity or shift their emission wavelengths in response to switching. Such changes permit the spatiotemporal control of fluorescence with optical stimulations and the implementation of imaging strategies that would be impossible to replicate with conventional fluorophores. Indeed, BODIPYs with photoactivatable fluorescence enable the selective highlighting of intracellular targets, the nanoscaled visualization of sub-cellular components, the real-time monitoring of dynamic events and the photochemical writing of optical barcodes.
硼二吡咯甲川（BODIPY）发色团是构建具有独特性质的光响应染料的多功能平台。具体来说，它与光可裂解基团的共价连接可用于设计具有光致开关荧光的化合物。所得的光活化荧光团可以响应开关而增加其发射强度或改变其发射波长。这种变化允许通过光学刺激对荧光进行时空控制，并实现传统荧光团不可能复制的成像策略。实际上，具有光活化荧光的 BODIPY 可以选择性地突出细胞内靶标，纳米级可视化亚细胞成分，实时监测动态事件以及光化学写入光学条形码。
