Yu Jing, Xu Jie, Ma Siyue, Wang Chao, Miao Qing, Wang Linlin, Chen Guang
Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China.
Chem Biomed Imaging. 2024 Apr 1;2(6):401-412. doi: 10.1021/cbmi.4c00006. eCollection 2024 Jun 24.
Biological gasotransmitters (small molecules of gases) play important roles in signal transduction mechanisms and disease treatments. Although a large number of small-molecule donors have been developed, visualizing the release of small molecules remains challenging. Owing to their unique optical properties, fluorophores have been widely applied in cellular imaging and tracking. Researchers have used various fluorophores to develop small-molecule donors with fluorescent activity for visualizing the release of small molecules and their related therapies. These include fluorophores and their derivatives such as boron-dipyrromethene (BODIPY), coumarin, 1,8-naphthalimide, hemicyanine, porphyrin, rhodamine, and fluorescein. In this review, we summarize the design concepts of functional fluorescent small-molecule donors in terms of different types of fluorophores. Then, we discuss how these donors release small molecules, and the imaging modalities and biomedical applications facilitated by their fluorescent properties. With the systematic discussion of these publications, we hope to provide useful references for the development of more practical, advanced fluorescent small-molecule donors in the future.
生物气体递质（气体小分子）在信号转导机制和疾病治疗中发挥着重要作用。尽管已经开发出大量小分子供体，但可视化小分子的释放仍然具有挑战性。由于其独特的光学性质，荧光团已广泛应用于细胞成像和追踪。研究人员使用各种荧光团开发具有荧光活性的小分子供体，以可视化小分子的释放及其相关治疗。这些包括荧光团及其衍生物，如硼二吡咯亚甲基（BODIPY）、香豆素、1,8-萘二甲酰亚胺、半菁、卟啉、罗丹明和荧光素。在这篇综述中，我们根据不同类型的荧光团总结了功能性荧光小分子供体的设计概念。然后，我们讨论这些供体如何释放小分子，以及它们的荧光性质所促进的成像方式和生物医学应用。通过对这些出版物的系统讨论，我们希望为未来开发更实用、先进的荧光小分子供体提供有用的参考。
