Faculty of Engineering and Applied Science, University of Ontario Institute of Technology, Ontario, Canada.
Faculty of Science, University of Ontario Institute of Technology, Ontario, Canada.
J Mech Behav Biomed Mater. 2021 Aug;120:104583. doi: 10.1016/j.jmbbm.2021.104583. Epub 2021 May 24.
This paper focuses on utilizing the Fused Deposition Modeling (FDM) to manufacture Polycaprolactone/Nano-Hydroxyapatite/Chitin-Nano-Whisker nanocomposite scaffolds and their subsequent characterization for biomedical applications. FDM nanocomposite filaments were manufactured in multiple nanocomposite formulations of Polycaprolactone/Nano-Hydroxyapatite (nHA), Polycaprolactone/Chitin-Nano-Whisker (CNW), and Polycaprolactone/nHA/CNW using a green method. The FDM processing conditions were optimized using Taguchi orthogonal array method. The mechanical, biodegradation, and biocompatibility properties of the bone tissue scaffolds were assessed. A preosteoblast mouse bone cell line was used for cell proliferation and attachment assays. The results indicated that CNW content in the filaments slightly increases the mechanical properties of the 3D printed parts, and the nanocomposite with 3% CNW content exhibited significant improvement in the cell proliferation and attachment properties of the scaffolds. The nHA content considerably improved the mechanical properties of the scaffolds. The nHA and CNW nanofillers increased the biodegradation rate of PCL. In general, considering all types of responses, a green manufactured nanocomposite of PCL/nHA/CNW can significantly increase the biological and mechanical properties of the 3D printed products for bone tissue scaffolds.
本文专注于利用熔丝制造技术（Fused Deposition Modeling，FDM）制造聚己内酯/纳米羟基磷灰石/壳聚糖纳米晶须纳米复合材料支架，并对其进行生物医学应用的后续特性研究。通过绿色方法，使用多组聚己内酯/纳米羟基磷灰石（nHA）、聚己内酯/壳聚糖纳米晶须（CNW）和聚己内酯/nHA/CNW 的纳米复合材料配方，制造了 FDM 纳米复合材料长丝。使用 Taguchi 正交数组方法优化了 FDM 加工条件。评估了骨组织支架的机械、生物降解和生物相容性特性。使用前成骨细胞小鼠骨细胞系进行细胞增殖和附着实验。结果表明，丝中的 CNW 含量略微提高了 3D 打印部件的机械性能，而含有 3%CNW 的纳米复合材料显著提高了支架的细胞增殖和附着性能。nHA 含量大大提高了支架的机械性能。nHA 和 CNW 纳米填料增加了 PCL 的生物降解率。总的来说，考虑到所有类型的响应，绿色制造的 PCL/nHA/CNW 纳米复合材料可以显著提高用于骨组织支架的 3D 打印产品的生物和机械性能。
