Institute of Polymeric Materials, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran; Department of Polymer Engineering, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran; Department of Mechanical Engineering, École de Technologie Supérieure, Université du Québec, Montréal, QC, Canada.
Institute of Polymeric Materials, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran; Department of Polymer Engineering, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran.
Int J Biol Macromol. 2021 Jul 1;182:1638-1649. doi: 10.1016/j.ijbiomac.2021.05.163. Epub 2021 May 27.
Chitosan (CS) and poly (ε-caprolactone) (PCL) are two most usable polymers in biomedical applications. In this study, chitosan has been modified and incorporated with poly (ε-caprolactone) to fabricate bone tissue engineering scaffold. Moreover, hydroxyapatite nanoparticles were added to enhance bioactivity and mechanical properties of scaffold. Bulk and fibrous comparative results showed significant effect of fiber diameter and distribution on mechanical properties. Moreover, the incorporation of chitosan-g-poly (ε-caprolactone) (CS-g-PCL) significantly decreases fiber diameter of pure PCL scaffold. Furthermore, both CS-g-PCL and nHA enhance mineralization and degradation of the scaffold soaked in simulated body fluid (SBF) and phosphate buffered saline (PBS), respectively. In vitro cytocompatibility assays also confirmed high cell viability and proliferation on the samples. Taken together, the results suggest that the microfabricated nanocomposite scaffolds could be used in bone tissue engineering.
壳聚糖（CS）和聚己内酯（PCL）是生物医学应用中最常用的两种聚合物。在这项研究中，壳聚糖经过改性并与聚己内酯结合，制备了骨组织工程支架。此外，还添加了羟基磷灰石纳米粒子来提高支架的生物活性和机械性能。块状和纤维状的比较结果表明纤维直径和分布对机械性能有显著影响。此外，壳聚糖接枝聚己内酯（CS-g-PCL）的加入显著降低了纯 PCL 支架的纤维直径。此外，CS-g-PCL 和 nHA 分别增强了在模拟体液（SBF）和磷酸盐缓冲盐水（PBS）中浸泡的支架的矿化和降解。体外细胞相容性试验也证实了样品上高细胞活力和增殖。综上所述，结果表明微制造的纳米复合材料支架可用于骨组织工程。
