Department of Gastroenterology, Hospital Universitari Mútua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain.
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Nutrients. 2021 May 15;13(5):1684. doi: 10.3390/nu13051684.
The study of intraepithelial lymphocytes (IEL) by flow cytometry is a useful tool in the diagnosis of coeliac disease (CD). Previous data showed that an increase in %TCRγδ and decrease of %CD3 IEL constitute a typical CD cytometric pattern with a specificity of 100%. However, there are no data regarding whether there are differences in the %TCRγδ related to sex, age, titers of serology, and degree of histological lesion.
To confirm the high diagnostic accuracy of the coeliac cytometric patterns. To determine if there are differences between sex, age, serology titers, and histological lesion grade.
We selected all patients who fulfilled "4 of 5" rule for CD diagnosis ( = 169). There were no differences in %TCRγδ between sexes ( = 0.909), age groups ( = 0.986), serology titers ( = 0.53) and histological lesion grades ( = 0.41). The diagnostic accuracy of complete CD cytometric pattern was: specificity 100%, sensitivity 82%, PPV 100%, NPV 47%.
We confirmed, in a validation cohort, the high diagnostic accuracy of complete CD pattern irrespective of sex, age, serology titers, and grade of mucosal lesion.
通过流式细胞术研究上皮内淋巴细胞（IEL）是诊断乳糜泻（CD）的有用工具。先前的数据表明，%TCRγδ 的增加和 %CD3 IEL 的减少构成了典型的 CD 流式细胞术模式，特异性为 100%。然而，关于性别、年龄、血清学滴度和组织学病变程度与 %TCRγδ 是否存在差异，尚无数据。
确认乳糜泻细胞术模式具有较高的诊断准确性。确定性别、年龄、血清学滴度和组织学病变分级之间是否存在差异。
我们选择了所有符合 CD 诊断“4 分 5 项”规则的患者（= 169）。%TCRγδ 在性别（= 0.909）、年龄组（= 0.986）、血清学滴度（= 0.53）和组织学病变分级（= 0.41）之间无差异。完整 CD 细胞术模式的诊断准确性为：特异性 100%，敏感性 82%，PPV 100%，NPV 47%。
我们在验证队列中证实，无论性别、年龄、血清学滴度和黏膜病变程度如何，完整的 CD 模式均具有较高的诊断准确性。
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